Construct: ORF TRCN0000472368
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011361.1_s317c1
- Derived from:
- ccsbBroadEn_12655
- DNA Barcode:
- AAGGAGGCGGTTGGACATCAGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCNX2 (80003)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472368
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80003 | PCNX2 | pecanex 2 | XM_006711816.3 | 21.8% | 21.8% | (many diffs) |
| 2 | human | 80003 | PCNX2 | pecanex 2 | XR_001737431.1 | 21.5% | 1_3009del;3895_4016del;4044_4236del | |
| 3 | human | 80003 | PCNX2 | pecanex 2 | XR_247042.3 | 21.4% | (many diffs) | |
| 4 | human | 80003 | PCNX2 | pecanex 2 | XM_011544278.2 | 21.4% | 21.3% | (many diffs) |
| 5 | human | 80003 | PCNX2 | pecanex 2 | XR_426919.3 | 21.3% | (many diffs) | |
| 6 | human | 80003 | PCNX2 | pecanex 2 | XR_001737430.1 | 21.2% | 1_3009del;3895_4062del;4090_4282del | |
| 7 | human | 80003 | PCNX2 | pecanex 2 | XR_001737432.2 | 17.3% | (many diffs) | |
| 8 | human | 80003 | PCNX2 | pecanex 2 | XR_001737429.1 | 15.8% | (many diffs) | |
| 9 | human | 80003 | PCNX2 | pecanex 2 | XM_017002391.1 | 15.4% | 15.3% | (many diffs) |
| 10 | human | 80003 | PCNX2 | pecanex 2 | NM_014801.4 | 13.9% | 13.9% | (many diffs) |
| 11 | human | 80003 | PCNX2 | pecanex 2 | XM_017002392.1 | 11.8% | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 978
- ORF length:
- 912
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gttttttggt ggttctgctg tgtctgggat aacctcggct gtttacagtg 121 tggcccggag cgtcttggct gccgccctgc tccacgcagt ctgcttcagt gcagtgaagg 181 aaccgtggag catgcaacac atcccggcac tgttttcggc cttctgtggc ctcttggtcg 241 ccctttctta ccatctgagc cgtcagagca gtgacccatc tgtactcatg tccttcatcc 301 aatgcaggct gtttcctaaa tttttacatc aaaatctggc agagtcagct gctgaccctc 361 tccccaagaa gatgaaagat tcagtgacgg atgtcttaaa atgggatctc atcgtctgcg 421 cagtggttgc tgtcctctca tttgcagtca gcgccagcac tgtattcctg tcattgcgac 481 catttctcag catcgtgctg tttgccttgg ctggagccgt ggggtttgta acacattacg 541 tgctccctca gctccgcaag catcatcCCT GGATGTGGAT TTCACACCCC ATTCTCAAAA 601 ACAAAGAGTA TCATCAACGG GAAGTGAGAG ATGTTGCCCA TTTAATGTGG TTCGAAAGAC 661 TCTATGTTTG GCTTCAGTGT TTTGAAAAAT ACATCTTGTA CCCAGCGCTA ATTTTGAATG 721 CCCTCACTAT TGATGCATTT TTAATAAGCA ATCACCGGAG ACTTGGTACC CACTGGGACA 781 TTTTTCTGAT GATCATTGCT GGCATGAAGC TGTTGCGGAC ATCATTCTGC AACCCGGTTT 841 ACCAGTTTAT TAACTTGAGC TTCACTGTCA TCTTTTTCCA CTTTGACTAC AAAGATATTT 901 CAGAGAGCTT CTTACTGGAT TTCTTCATGG TGTCCATTTT ATTTAGCAAG GTGTTTCTGG 961 GCTACGTGAG GCAAAAATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1021 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1081 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAAGGAGG CGGTTGGACA 1141 TCAGCGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt