Construct: ORF TRCN0000472369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001207.1_s317c1
- Derived from:
- ccsbBroadEn_09046
- DNA Barcode:
- AGCTAGCCCCGTTATGCTTATCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDFIP1 (80762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80762 | NDFIP1 | Nedd4 family interacting pr... | NM_030571.4 | 99.8% | 99.5% | 32T>G |
| 2 | mouse | 65113 | Ndfip1 | Nedd4 family interacting pr... | NM_022996.1 | 93.2% | 96.8% | (many diffs) |
| 3 | mouse | 65113 | Ndfip1 | Nedd4 family interacting pr... | XM_006526149.1 | 93% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gttggcgttg gcggcgctgg cggcgggcga gccggcctgc ggcagccggt 121 accagcagtt gcagaatgaa gaagagtctg gagaacctga acaggctgca ggtgatgctc 181 ctccacctta cagcagcatt tctgcagaga gcgcagcata ttttgactac aaggatgagt 241 ctgggtttcc aaagccccca tcttacaatG TAGCTACAAC ACTGCCCAGT TATGATGAAG 301 CGGAGAGGAC CAAGGCTGAA GCTACTATCC CTTTGGTTCC TGGGAGAGAT GAGGATTTTG 361 TGGGTCGGGA TGATTTTGAT GATGCTGACC AGCTGAGGAT AGGAAATGAT GGGATTTTCA 421 TGTTAACTTT TTTCATGGCA TTCCTCTTTA ACTGGATTGG GTTTTTCCTG TCTTTTTGCC 481 TGACCACTTC AGCTGCAGGA AGGTATGGGG CCATTTCAGG ATTTGGTCTC TCTCTAATTA 541 AATGGATCCT GATTGTCAGG TTTTCCACCT ATTTCCCTGG ATATTTTGAT GGTCAGTACT 601 GGCTCTGGTG GGTGTTCCTT GTTTTAGGCT TTCTCCTGTT TCTCAGAGGA TTTATCAATT 661 ATGCAAAAGT TCGGAAGATG CCAGAAACTT TCTCAAATCT CCCCAGGACC AGAGTTCTCT 721 TTATTTATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCTAG CCCCGTTATG CTTATCCAAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt