Construct: ORF TRCN0000472380
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012336.1_s317c1
- Derived from:
- ccsbBroadEn_13528
- DNA Barcode:
- GGTTCGCAAGGGAGCGTAGCGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VSTM1 (284415)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472380
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284415 | VSTM1 | V-set and transmembrane dom... | XM_017026666.1 | 100% | 100% | |
| 2 | human | 284415 | VSTM1 | V-set and transmembrane dom... | NM_001288792.1 | 75.2% | 60% | (many diffs) |
| 3 | human | 284415 | VSTM1 | V-set and transmembrane dom... | NM_198481.4 | 72.8% | 69.4% | (many diffs) |
| 4 | human | 284415 | VSTM1 | V-set and transmembrane dom... | XM_011526849.1 | 65.4% | 46.8% | 393_460del;591_798del |
| 5 | human | 284415 | VSTM1 | V-set and transmembrane dom... | XM_011526847.1 | 53% | 39.7% | (many diffs) |
| 6 | human | 284415 | VSTM1 | V-set and transmembrane dom... | XM_011526848.1 | 51.9% | 42% | (many diffs) |
| 7 | human | 284415 | VSTM1 | V-set and transmembrane dom... | XM_011526846.1 | 51.4% | 49.1% | (many diffs) |
| 8 | human | 284415 | VSTM1 | V-set and transmembrane dom... | NR_110142.2 | 48.8% | (many diffs) | |
| 9 | human | 284415 | VSTM1 | V-set and transmembrane dom... | XR_935808.2 | 48.3% | 1_189del;541_834del;1006_1080del | |
| 10 | human | 284415 | VSTM1 | V-set and transmembrane dom... | XM_011526845.1 | 47.8% | 35.4% | 352_645del;687_754del;885_1092del |
| 11 | human | 284415 | VSTM1 | V-set and transmembrane dom... | NM_001288791.2 | 33.5% | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 591
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgcagaa ttcctctccc tgctttgcct cgGGCTGTGT CTGGGCTACG 121 AAGATGAGAA AAAGAATGAG AAACCGCCCA AGCCCTCCCT CCACGCCTGG CCCAGCTCGG 181 TGGTTGAAGC CGAGAGCAAT GTGACCCTGA AGTGTCAGGC TCATTCCCAG AATGTGACAT 241 TTGTGCTGCG CAAGGTGAAC GACTCTGGGT ACAAGCAGGA ACAGAGCTCG GCAGAAAACG 301 AAGCTGAATT CCCCTTCACG GACCTGAAGC CTAAGGATGC TGGGAGGTAC TTTTGTGCCT 361 ACAAGACAAC AGCCTCCCAT GAGTGGTCAG AAAGCAGTGA ACACTTGCAG CTGGTGGTCA 421 CAGATAAACA CGATGAACTT GAAGCTCCCT CAATGAAAAC AGACACCAGA ACCATCTTTG 481 TCGCCATCTT CAGCTGCATC TCCATCCTTC TCCTCTTCCT CTCAGTCTTC ATCATCTACA 541 GATGCAGCCA GCACAGTGAG CTCAGAGAAC GCAAAGGGAG AGAGGGGGAG TTGCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGAGGTT CGCAAGGGAG CGTAGCGTGA ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt