Construct: ORF TRCN0000472403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000905.1_s317c1
- Derived from:
- ccsbBroadEn_02706
- DNA Barcode:
- TTCCATCTCTTCGTTCTGGCGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DKK1 (22943)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22943 | DKK1 | dickkopf WNT signaling path... | NM_012242.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 864
- ORF length:
- 798
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggctctgggc gcagcgggag ctacccgggt ctttgtcgcg atggtagcgg 121 cggctctcgg cggccaccct ctgctgggag tgagcgccac cttgaactcg gttctcaatt 181 ccaacgctat caagaacctg cccccaccgc tgggcggcgc tgcggggcac ccaggctctg 241 cagtcagcgc cgcgccggga atcctgtacc cgggcgggaa taagtaccag accattgaca 301 actaccagcc gtacccgtgc gcagaggacg aggagtgcgg cactgatgag tactgcgcta 361 gtcccacccg cggaggggac gcaggcgtgc aaatctgtct cgcctgcagg aagcgccgaa 421 aacgctgcat gcgtcacgct atgtgctgcc ccgggaatta ctgcaaaaat ggaatatgtg 481 tgtcttctga tcaaaatcat ttccgaggag aaattgagga aaccatcact gaaagctttg 541 gtaatgatca tagcaccttG GATGGGTATT CCAGAAGAAC CACCTTGTCT TCAAAAATGT 601 ATCACACCAA AGGACAAGAA GGTTCTGTTT GTCTCCGGTC ATCAGACTGT GCCTCAGGAT 661 TGTGTTGTGC TAGACACTTC TGGTCCAAGA TCTGTAAACC TGTCCTGAAA GAAGGTCAAG 721 TGTGTACCAA GCATAGGAGA AAAGGCTCTC ATGGACTAGA AATATTCCAG CGTTGTTACT 781 GTGGAGAAGG TCTGTCTTGC CGGATACAGA AAGATCACCA TCAAGCCAGT AATTCTTCTA 841 GGCTTCACAC TTGTCAGAGA CACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TCCATCTCTT 1021 CGTTCTGGCG CGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att