Construct: ORF TRCN0000472404

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007107.1_s317c1
Derived from:
ccsbBroadEn_15721
DNA Barcode:
GCCAATTATATCATGCGGCATTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYL12A (10627)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472404
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10627 MYL12A myosin light chain 12A NM_001303047.1 99.6% 98.8% 320C>T;509A>C
2 human 10627 MYL12A myosin light chain 12A NM_001303048.1 99.6% 98.8% 320C>T;509A>C
3 human 10627 MYL12A myosin light chain 12A NM_006471.3 99.6% 98.8% 320C>T;509A>C
4 human 10627 MYL12A myosin light chain 12A NM_001303049.1 96.2% 95.4% 1_18del;338C>T;527A>C
5 human 103910 MYL12B myosin light chain 12B NM_001144944.1 94.7% 96.5% (many diffs)
6 human 103910 MYL12B myosin light chain 12B NM_001144945.1 94.7% 96.5% (many diffs)
7 human 103910 MYL12B myosin light chain 12B NM_033546.3 94.7% 96.5% (many diffs)
8 human 10398 MYL9 myosin light chain 9 NM_006097.5 77.9% 91.8% (many diffs)
9 human 10398 MYL9 myosin light chain 9 NM_181526.3 54.9% 63.3% (many diffs)
10 mouse 67938 Myl12b myosin, light chain 12B, re... NM_023402.2 86% 96.5% (many diffs)
11 mouse 67268 Myl12a myosin, light chain 12A, re... NM_026064.2 85% 96.5% (many diffs)
12 mouse 67268 Myl12a myosin, light chain 12A, re... XM_006524822.1 82.2% 93.2% (many diffs)
13 mouse 98932 Myl9 myosin, light polypeptide 9... NM_172118.1 80.6% 92.4% (many diffs)
14 mouse 98932 Myl9 myosin, light polypeptide 9... XM_006500453.3 80.6% 92.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
579
ORF length:
513
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gagcaaaaga acaaagacca agaccaagaa gcgccctcag cgtgcaacat
121ccaatgtgtt tgctatgttt gaccagtcac agattcagga gttcaaagag gccttcaaca
181tgattgatca gaaCAGAGAT GGTTTCATCG ACAAGGAAGA TTTGCATGAT ATGCTTGCTT
241CATTGGGGAA GAATCCAACT GATGAGTATC TAGATGCCAT GATGAATGAG GCTCCAGGCC
301CCATCAATTT CACCATGTTC CTCACCATGT TTGGTGAGAA GTTAAATGGC ACAGATCCTG
361AAGATGTCAT CAGAAATGCC TTTGTTTGCT TTGATGAAGA AGCAACTGGC ACCATACAGG
421AAGATTACTT GAGAGAGCTG CTGACAACCA TGGGGGATCG GTTTACAGAT GAGGAAGTGG
481ATGAGCTGTA CAGAGAAGCA CCTATTGATA AAAAGGGGAA TTTCAATTAC ATCGAGTTCA
541CACGCATCCT GAAACATGGA GCCAAAGACA AAGCTGACTG CCCAACTTTC TTGTACAAAG
601TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
661GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
721ACGAGCCAAT TATATCATGC GGCATTGCAC GCGTTAAGTC gacaatcaac ctctggatta
781caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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