Construct: ORF TRCN0000472445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017712.1_s317c1
- Derived from:
- ccsbBroadEn_06636
- DNA Barcode:
- TCGAGCCCCGGTTATTTTCGTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFIC (4782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4782 | NFIC | nuclear factor I C | NM_005597.4 | 99.9% | 99.7% | 1276T>G |
| 2 | human | 4782 | NFIC | nuclear factor I C | XM_017026836.2 | 97.6% | 97.4% | 0_1ins28;2delT;1249T>G |
| 3 | human | 4782 | NFIC | nuclear factor I C | NM_001245004.2 | 96.5% | 96.3% | 1270_1276delCCTACTC;1280_1311del;1317_1318insCTGGGA |
| 4 | human | 4782 | NFIC | nuclear factor I C | XM_017026835.2 | 96.5% | 96.3% | 1270_1276delCCTACTC;1280_1311del;1317_1318insCTGGGA |
| 5 | human | 4782 | NFIC | nuclear factor I C | NM_001245005.2 | 94.4% | 94.1% | (many diffs) |
| 6 | human | 4782 | NFIC | nuclear factor I C | NM_001245002.2 | 83.3% | 83.4% | (many diffs) |
| 7 | human | 4782 | NFIC | nuclear factor I C | XM_005259564.4 | 82.8% | 82.9% | (many diffs) |
| 8 | human | 4782 | NFIC | nuclear factor I C | NM_205843.3 | 81.5% | 81.4% | (many diffs) |
| 9 | human | 4782 | NFIC | nuclear factor I C | XM_017026834.2 | 75% | 75% | (many diffs) |
| 10 | human | 4782 | NFIC | nuclear factor I C | XM_006722759.2 | 73.3% | 73.2% | (many diffs) |
| 11 | mouse | 18029 | Nfic | nuclear factor I/C | NM_026756.2 | 89.3% | 96.2% | (many diffs) |
| 12 | mouse | 18029 | Nfic | nuclear factor I/C | NM_008688.3 | 86.9% | 92.9% | (many diffs) |
| 13 | mouse | 18029 | Nfic | nuclear factor I/C | XM_006513335.3 | 84.9% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1350
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ttcgtccccg ctctgcctca cccaggatga gttccacccg ttcatcgagg 121 ccctgctgcc tcacgtccgc gccttcgcct acacctggtt caacctgcag gcgcggaagc 181 gcaagtactt caagaagcac gagaagcgga tgtcgaagga cgaggagcgt gcggtcaagg 241 acgagctgct gggcgagaag cccgaggtca agcagaagtg ggcgtcgcgg ctgctggcca 301 agctgcgcaa ggacatccgg cccgagtgcc gcgaggactt cgtgctgagc atcaccggca 361 agaaggcgcc gggctgcgtg ctctccaacc ccgaccagaa gggcaagatg cggcgcatcg 421 actgtctccg gcaggcggac aaggtgtggc ggctggacct ggtcatggtc atcctgttca 481 agggcatccc gctggagagc accgacggcg agcgcctggt caaggctgcg cagtgcggtc 541 acccggtcct gtgcgtgcag ccgcaccaca ttggcgtggc cgtcaaggag ctggacctct 601 acctggccta cttcgtgcgt gagcgagatg cagagcaaag cggcagtccc cggacaggga 661 tgggctctga ccaggaggac agcaagccca tcacgctgga cacgaccgac ttccaggaga 721 gctttgtcac ctccggcgtg ttcagcgtca ctgagctcat ccaagtgtcc cggacacccg 781 tggtgactgg aacaggaccc aacttctccc tgggggagct gcaggggcac ctggcatacg 841 acctgaaccc agccagcact ggccTCAGAA GAACGCTGCC CAGCACCTCC TCCAGTGGGA 901 GCAAGCGGCA CAAATCGGGC TCGATGGAGG AAGACGTGGA CACGAGCCCT GGCGGCGATT 961 ACTACACTTC GCCCAGCTCG CCCACGAGTA GCAGCCGCAA CTGGACGGAG GACATGGAAG 1021 GAGGCATCTC GTCCCCGGTG AAGAAGACAG AGATGGACAA GTCACCATTC AACAGCCCGT 1081 CCCCCCAGGA CTCTCCCCGC CTCTCCAGCT TCACCCAGCA CCACCGGCCC GTCATCGCCG 1141 TGCACAGCGG GATCGCCCGG AGCCCACACC CGTCCTCCGC TCTGCATTTC CCTACGACGT 1201 CCATCCTACC CCAGACGGCC TCCACCTACT TCCCCCACAC GGCCATCCGC TACCCACCTC 1261 ATCTCAACCC CCAGGACCCG CTCAAAGATC TTGTCTCGCT GGCCTGCGAC CCAGCCAGCC 1321 AGCAACCTGG ACCGTCCTGG GATCTGGGAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCGAG 1501 CCCCGGTTAT TTTCGTCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt