Construct: ORF TRCN0000472455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004731.1_s317c1
- Derived from:
- ccsbBroadEn_04322
- DNA Barcode:
- CTCAGCTTGAGGGTTCCTGGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDHD2 (84064)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472455
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | NM_032124.5 | 100% | 100% | |
| 2 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_011526227.2 | 100% | 100% | |
| 3 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_011526228.3 | 100% | 100% | |
| 4 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_017026039.1 | 100% | 100% | |
| 5 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_005258371.4 | 88.8% | 88% | (many diffs) |
| 6 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_024451272.1 | 88.8% | 88% | (many diffs) |
| 7 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | NM_001318765.2 | 65.2% | 65.2% | 0_1ins270 |
| 8 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_006722557.2 | 65.2% | 65.2% | 0_1ins270 |
| 9 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_017026040.1 | 54.4% | 53.2% | (many diffs) |
| 10 | mouse | 76987 | Hdhd2 | haloacid dehalogenase-like ... | NM_001039201.1 | 86.5% | 89.1% | (many diffs) |
| 11 | mouse | 76987 | Hdhd2 | haloacid dehalogenase-like ... | NM_029826.2 | 86.5% | 89.1% | (many diffs) |
| 12 | mouse | 76987 | Hdhd2 | haloacid dehalogenase-like ... | XM_006526442.3 | 86.5% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcatgccgt gcattaaaag ctgttttggt agatctcagt ggcacacttc 121 acattgaaga tgcagctgtg ccaggcgcac aggaagctct taaaaggtta cgtggtgctt 181 ctgtaatcat taggtttgtg accaatacaa ccaaagagag caagcaagac ctgttagaaa 241 ggttgagaaa attggaattt gatatctctg aagatgaaat attcacatcT CTGACTGCAG 301 CCAGAAGTTT ACTAGAGCGG AAACAAGTCA GACCCATGCT GCTAGTTGAT GATCGGGCAC 361 TACCTGATTT CAAAGGAATA CAAACAAGTG ATCCTAATGC TGTGGTCATG GGATTGGCAC 421 CAGAACATTT TCATTATCAA ATTCTGAATC AAGCATTCCG GTTACTCCTG GATGGAGCAC 481 CTCTGATAGC AATCCACAAA GCCAGGTATT ACAAGAGGAA AGATGGCTTA GCCCTGGGGC 541 CTGGACCATT TGTGACTGCT TTAGAGTATG CCACAGATAC CAAAGCCACA GTCGTGGGGA 601 AACCAGAGAA GACGTTCTTT TTGGAAGCAT TGCGGGGCAC TGGCTGTGAA CCTGAGGAGG 661 CTGTCATGAT AGGAGATGAT TGCAGGGATG ATGTTGGTGG GGCTCAAGAT GTCGGCATGC 721 TGGGCATCTT AGTAAAGACT GGGAAATATC GAGCATCAGA TGAAGAAAAA ATTAATCCAC 781 CTCCTTACTT AACTTGTGAG AGTTTCCCTC ATGCTGTGGA CCACATTCTG CAGCACCTAT 841 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGACT CAGCTTGAGG GTTCCTGGAT ACACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt