Construct: ORF TRCN0000472460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014961.1_s317c1
- Derived from:
- ccsbBroadEn_14961
- DNA Barcode:
- CAGTTATAATCGGCCCACAAAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGB1P1 (10357)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3146 | HMGB1 | high mobility group box 1 | NM_001313892.1 | 98.9% | 98.1% | (many diffs) |
| 2 | human | 3146 | HMGB1 | high mobility group box 1 | NM_001313893.1 | 98.9% | 98.1% | (many diffs) |
| 3 | human | 3146 | HMGB1 | high mobility group box 1 | NM_001370340.1 | 98.7% | 98.1% | (many diffs) |
| 4 | human | 3146 | HMGB1 | high mobility group box 1 | NM_001370341.1 | 98.7% | 98.1% | (many diffs) |
| 5 | human | 3146 | HMGB1 | high mobility group box 1 | NM_002128.7 | 98.7% | 98.1% | (many diffs) |
| 6 | human | 3146 | HMGB1 | high mobility group box 1 | XM_024449341.1 | 98.7% | 98.1% | (many diffs) |
| 7 | human | 3146 | HMGB1 | high mobility group box 1 | NM_001363661.1 | 72% | 71.1% | (many diffs) |
| 8 | human | 3146 | HMGB1 | high mobility group box 1 | NM_001370339.1 | 72% | 71.1% | (many diffs) |
| 9 | human | 6672 | SP100 | SP100 nuclear antigen | NM_003113.4 | 20.6% | 18% | (many diffs) |
| 10 | mouse | 100862258 | Gm21596 | predicted gene, 21596 | XM_003945339.3 | 91% | 97.2% | (many diffs) |
| 11 | mouse | 15289 | Hmgb1 | high mobility group box 1 | NM_001313894.1 | 90.8% | 97.2% | (many diffs) |
| 12 | mouse | 15289 | Hmgb1 | high mobility group box 1 | NM_010439.4 | 90.8% | 97.2% | (many diffs) |
| 13 | mouse | 628431 | Hmgb1-rs17 | high mobility group box 1, ... | NR_033589.1 | 37.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 714
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaaagga gatcctaaga agccgagagg caaaatgtca tcatatgcat 121 tttttgtgca aacttgtcgg gaggagcata agaagaagca cccagatgct tcggtcaact 181 tctcagagtt ttctaagaag tgctcagaga ggtggaagac catgtctgct aaagagaaag 241 gaaaatttga ggatatggca aaggcggaca agacccatta tgaaagacaa atgaaaacct 301 atatccctcc caaagggGAG ACAAAAAAGA AGTTCAAGGA TCCCAATGCA CCCAAGAGGC 361 CTCCTTCGGC CTTCTTCCTC TTCTGCTCTG AGTATCGCCC AAAAATCAAA GGAGAACATC 421 CTGGCCTGTC CATTGGTGAT GTTGCGAAGA AACTGGGAGA GATGTGGAAT AACACTGCTG 481 CAGATGACAA GCAGCATTAT GAAAAGAAGG CTGCGAAGCT GAAGGAAAAA TACGAAAAGG 541 ATATTGCTGC ATATCGAGCT AAAGGAAAGC CTGATGCAGC AAAAAAGGGA GTTGTCAAGG 601 CTGAAAAAAG CAAGAAAAAG AAGGAAGAGG AGGAAGATGA GGAAGATGAA GAGGATGAGG 661 AGGAGGAGGA AGATGAAGAA GATGAAGATG AAGAAGAAGA TGATGATGAT GAATTGCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAC AGTTATAATC GGCCCACAAA GCCACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att