Construct: ORF TRCN0000472460

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014961.1_s317c1
Derived from:
ccsbBroadEn_14961
DNA Barcode:
CAGTTATAATCGGCCCACAAAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HMGB1P1 (10357)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472460
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3146 HMGB1 high mobility group box 1 NM_001313892.1 98.9% 98.1% (many diffs)
2 human 3146 HMGB1 high mobility group box 1 NM_001313893.1 98.9% 98.1% (many diffs)
3 human 3146 HMGB1 high mobility group box 1 NM_001370340.1 98.7% 98.1% (many diffs)
4 human 3146 HMGB1 high mobility group box 1 NM_001370341.1 98.7% 98.1% (many diffs)
5 human 3146 HMGB1 high mobility group box 1 NM_002128.7 98.7% 98.1% (many diffs)
6 human 3146 HMGB1 high mobility group box 1 XM_024449341.1 98.7% 98.1% (many diffs)
7 human 3146 HMGB1 high mobility group box 1 NM_001363661.1 72% 71.1% (many diffs)
8 human 3146 HMGB1 high mobility group box 1 NM_001370339.1 72% 71.1% (many diffs)
9 human 6672 SP100 SP100 nuclear antigen NM_003113.4 20.6% 18% (many diffs)
10 mouse 100862258 Gm21596 predicted gene, 21596 XM_003945339.3 91% 97.2% (many diffs)
11 mouse 15289 Hmgb1 high mobility group box 1 NM_001313894.1 90.8% 97.2% (many diffs)
12 mouse 15289 Hmgb1 high mobility group box 1 NM_010439.4 90.8% 97.2% (many diffs)
13 mouse 628431 Hmgb1-rs17 high mobility group box 1, ... NR_033589.1 37.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
714
ORF length:
645
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggcaaagga gatcctaaga agccgagagg caaaatgtca tcatatgcat
121tttttgtgca aacttgtcgg gaggagcata agaagaagca cccagatgct tcggtcaact
181tctcagagtt ttctaagaag tgctcagaga ggtggaagac catgtctgct aaagagaaag
241gaaaatttga ggatatggca aaggcggaca agacccatta tgaaagacaa atgaaaacct
301atatccctcc caaagggGAG ACAAAAAAGA AGTTCAAGGA TCCCAATGCA CCCAAGAGGC
361CTCCTTCGGC CTTCTTCCTC TTCTGCTCTG AGTATCGCCC AAAAATCAAA GGAGAACATC
421CTGGCCTGTC CATTGGTGAT GTTGCGAAGA AACTGGGAGA GATGTGGAAT AACACTGCTG
481CAGATGACAA GCAGCATTAT GAAAAGAAGG CTGCGAAGCT GAAGGAAAAA TACGAAAAGG
541ATATTGCTGC ATATCGAGCT AAAGGAAAGC CTGATGCAGC AAAAAAGGGA GTTGTCAAGG
601CTGAAAAAAG CAAGAAAAAG AAGGAAGAGG AGGAAGATGA GGAAGATGAA GAGGATGAGG
661AGGAGGAGGA AGATGAAGAA GATGAAGATG AAGAAGAAGA TGATGATGAT GAATTGCCAA
721CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
781ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
841TATCTTGTGG AAAGGACGAC AGTTATAATC GGCCCACAAA GCCACGCGTT AAGTCgacaa
901tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)