Construct: ORF TRCN0000472471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009757.1_s317c1
- Derived from:
- ccsbBroadEn_06558
- DNA Barcode:
- AGAGGTACCCTTCCATCGGTGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAOB (4129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4129 | MAOB | monoamine oxidase B | NM_000898.5 | 99.8% | 99.6% | 113G>A;389C>A |
| 2 | human | 4129 | MAOB | monoamine oxidase B | XM_005272608.3 | 96.7% | 96.5% | 0_1ins48;65G>A;341C>A |
| 3 | human | 4129 | MAOB | monoamine oxidase B | XM_017029523.1 | 96.7% | 96.5% | 0_1ins48;65G>A;341C>A |
| 4 | human | 4129 | MAOB | monoamine oxidase B | XM_017029524.2 | 96.7% | 96.5% | 0_1ins48;65G>A;341C>A |
| 5 | mouse | 109731 | Maob | monoamine oxidase B | NM_172778.2 | 87.2% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1626
- ORF length:
- 1560
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caacaaatgc gacgtggtcg tggtgggggg cggcatctca ggtatggcag 121 cagccaaact tctgcatgac tctggactga atgtggttgt tctggaagcc cgggaccatg 181 tgggaggcag gacttacact cttaggaacc aaaaggttaa atatgtggac cttggaggat 241 cctatgttgg accaacccag aatcgtatct tgagattagc caaggagcta ggattggaga 301 cctacaaagt gaatgaggtt gagcgtctga tccaccatgt aaagggcaaa tcatacccct 361 tcagggggcc attcccacct gtatggaatc caattaccta cttagatcat aacaactttt 421 ggaggacaat ggatgacatg gggcgagaga ttcagagtga tgccccatgg aaggctcccc 481 ttgcagaaga gtgggacaac atgacaatga aggagctact ggacaagctc tgctggactg 541 aatctgcaaa gcagcttgcc actctctttg tgaacctgtg tgtcactgca gagacccatg 601 aggtctctgc tctctggttc ctgtggtatg tgaagcagtg tggaggcaca acaagaatca 661 tctcgacaac aaatggagga caggagagga aatttgtggg cggatctggt caagtgagtg 721 agcggataat ggacctcctt ggagaccgag tgaagctgga gaggcctgtg atctacattg 781 accagacaag agaaaatgtc cttgtggaga ccctaaacca tgagatgtat gaggctaaat 841 atgtgattag tgctattcct cctactctgg gcatgaagat tcacttcaat ccccctctgc 901 caatgatgag aaaccagatg atcactcgtg tgcctttggg ttcagtcatc aagtgtatag 961 tttattataa agagcctttc tggaggaaaa aggattactg tggaaccatg attattgatg 1021 gagaagaagc tccagttgcc tacacgttgg atgataccaa acctgaaggc aactatgctg 1081 ccataatggg atttatcctg gcccacaaag ccagaaaact ggCACGTCTT ACCAAAGAGG 1141 AAAGGTTGAA GAAACTTTGT GAACTCTATG CCAAGGTTCT GGGTTCCCTA GAAGCTCTGG 1201 AGCCAGTGCA TTATGAAGAA AAGAACTGGT GTGAGGAGCA GTACTCTGGG GGCTGCTACA 1261 CAACTTATTT CCCCCCTGGG ATCCTGACTC AATATGGAAG GGTTCTACGC CAGCCAGTGG 1321 ACAGGATTTA CTTTGCAGGC ACCGAGACTG CCACACACTG GAGCGGCTAC ATGGAGGGGG 1381 CTGTAGAGGC CGGGGAGAGA GCAGCCCGAG AGATCCTGCA TGCCATGGGG AAGATTCCAG 1441 AGGATGAAAT CTGGCAGTCA GAACCAGAGT CTGTGGATGT CCCTGCACAG CCCATCACCA 1501 CCACCTTTTT GGAGAGACAT TTGCCCTCCG TGCCAGGCCT GCTCAGGCTG ATTGGATTGA 1561 CCACCATCTT TTCAGCAACG GCTCTTGGCT TCCTGGCCCA CAAAAGGGGG CTACTTGTGA 1621 GAGTCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1681 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1741 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAGAGGTACC CTTCCATCGG TGAAGACGCG 1801 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt