Construct: ORF TRCN0000472479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017858.1_s317c1
- Derived from:
- ccsbBroadEn_00124
- DNA Barcode:
- CGCTATTCGTAGTGCAGTTTGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP1B3 (483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 483 | ATP1B3 | ATPase Na+/K+ transporting ... | NM_001679.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gaagaacgag aagaagtccc tcaaccagag cctggccgag tggaagctct 121 tcatctacaa cccgaccacc ggagaattcc tggggcgcac cgccaagagc tggggtttga 181 tcttgctctt ctacctagtt ttttatgggt tcctggctgc actcttctca ttcacgatgt 241 gggttatgct tcagactctc aacgatgagg ttccaaaata ccgtgaccag attcctagcc 301 caggactcat ggtttttcca aaaccagtga ccgcattgga atatacattc agtaggtctg 361 atccaacttc gtatgcaggg tacattgaag accttaagaa gtttctaaaa ccatatactt 421 tagaagaaca gaagaacctc acagtctgtc ctgatggagc actttttgaa cagaagggtc 481 cagtttatgt tgcatgtcag tttcctattt cattacttca agcatgcagt ggtatgaatg 541 atcctgattt tggctattct caaggaaacc cttgtattct tgtgaaaatg aacagaataa 601 ttggattaaa gcctgaagga gtgccaagga tagattgtgt ttcaaagaat gaagatatac 661 caaatgtagc agtttatcct cataatggaa tgatagactt aaaatatttc ccatattatg 721 ggaaaaaact gcatgttggg tatctacagc cattggttgc tgttcaggtc agctttgctc 781 ctaacaacaC TGGGAAAGAA GTAACAGTTG AGTGCAAGAT TGATGGATCA GCCAACCTAA 841 AAAGTCAGGA TGATCGTGAC AAGTTTTTGG GACGAGTTAT GTTCAAAATC ACAGCACGTG 901 CATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGACG CTATTCGTAG TGCAGTTTGG GAACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt