Construct: ORF TRCN0000472481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002039.1_s317c1
- Derived from:
- ccsbBroadEn_07667
- DNA Barcode:
- TATCATCGGATCTAACTATCCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CGRRF1 (10668)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472481
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10668 | CGRRF1 | cell growth regulator with ... | NM_006568.3 | 99.8% | 99.6% | 308G>A |
| 2 | human | 10668 | CGRRF1 | cell growth regulator with ... | XM_011536362.2 | 81.8% | 81.6% | 0_1ins180;128G>A |
| 3 | human | 10668 | CGRRF1 | cell growth regulator with ... | XM_017020931.1 | 81.8% | 81.6% | 0_1ins180;128G>A |
| 4 | human | 10668 | CGRRF1 | cell growth regulator with ... | XM_017020932.1 | 81.8% | 81.6% | 0_1ins180;128G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcggtgttt ctggtaacgc tttatgaata ctcgccgctt ttctacatcg 121 cggtggtctt tacctgcttc atcgtgacca ccggcctggt attgggatgg tttggttggg 181 atgttccagt aattctgaga aattcagaag agacccagtt cagcacaaga gttttcaaaa 241 agcaaatgag acaagtcaag aatccttttg gcttagagat cactaatcca tcttcagctt 301 caattacaac tggcataacc ttgacaacag attgccttga agatagcctc cttacatgct 361 actgggggtg caatgttcaa aaattatatg aagctctgca gaagcatgtt tattgcttca 421 gaataagcac tccccaagca ttagaagatg ctctgtatag tgaatatctc tatcaggaac 481 agtattttat taaaaaggat agcaaagaag aaatatattg ccagttacca agagatacta 541 aaattgaaga ctttggtaca gtacccagat ctcgctatcc attggtagcg ctattgacct 601 tagctgatga ggatgaccgg gaaatttatg atattatttc catGGTGTCA GTGATTCATA 661 TTCCTGATAG GACTTATAAA CTATCCTGCA GAATATTGTA TCAATATTTA CTCTTGGCTC 721 AAGGTCAATT TCATGATCTT AAGCAACTTT TCATGTCTGC AAATAATAAT TTCACTCCCT 781 CCAACAATTC CTCTTCAGAA GAAAAAAACA CAGACAGAAG TTTGTTGGAA AAGGTGGGAC 841 TCTCTGAAAG TGAAGTTGAG CCATCGGAAG AGAACAGCAA GGACTGTGTT GTTTGCCAGA 901 ATGGGACTGT GAACTGGGTA CTCTTACCAT GCAGACACAC ATGCCTGTGT GATGGCTGTG 961 TGAAGTATTT TCAGCAGTGC CCAATGTGCA GGCAGTTTGT TCAGGAATCT TTTGCACTTT 1021 GCAGTCAAAA AGAGCAAGAT AAAGACAAAC CGAAGACTCT TTGCCCAACT TTCTTGTACA 1081 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1141 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1201 AGGACGATAT CATCGGATCT AACTATCCCC CACGCGTTAA GTCgacaatc aacctctgga 1261 ttacaaaatt tgtgaaagat t