Construct: ORF TRCN0000472488
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002295.1_s317c1
- Derived from:
- ccsbBroadEn_01569
- DNA Barcode:
- ACGACCGGAAGAGAGCGCAAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRPB2 (6629)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472488
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6629 | SNRPB2 | small nuclear ribonucleopro... | NM_003092.5 | 100% | 100% | |
| 2 | human | 6629 | SNRPB2 | small nuclear ribonucleopro... | NM_198220.3 | 100% | 100% | |
| 3 | mouse | 20639 | Snrpb2 | U2 small nuclear ribonucleo... | NM_021335.3 | 89.6% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 741
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tatcagacca aatcatacaa tttatatcaa caatatgaat gacaaaatta 121 aaaaggaaga attgaagaga tccctatatg ccctgttttc tcagtttggt catgtggtgg 181 acattgtggc tttaaagacc atgaagatga gggggcaggc ctttgtcata tttaaggaac 241 tgggctcatc cacaaatgcc ttgagacagc tacaaggatt tccattttat ggtaaaccaa 301 tgcgaataca gtatgcaaaa acagattcGG ATATAATATC AAAAATGCGT GGAACTTTTG 361 CTGACAAAGA AAAGAAAAAA GAAAAGAAAA AAGCCAAAAC TGTGGAACAG ACTGCAACAA 421 CCACAAACAA AAAGCCTGGC CAGGGAACTC CAAATTCAGC TAATACCCAA GGAAATTCAA 481 CACCAAATCC TCAGGTCCCT GATTACCCTC CAAACTATAT TTTATTCCTT AATAACTTAC 541 CAGAAGAGAC TAATGAGATG ATGTTATCCA TGCTGTTTAA TCAGTTCCCT GGCTTCAAGG 601 AAGTACGTCT GGTACCAGGG AGGCATGACA TTGCTTTTGT TGAATTTGAA AATGATGGGC 661 AGGCTGGAGC TGCCAGGGAT GCTTTACAGG GATTTAAGAT CACACCGTCC CATGCTATGA 721 AGATCACCTA TGCCAAGAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACGA CCGGAAGAGA 901 GCGCAAATTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt