Construct: ORF TRCN0000472502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018970.3_s317c1
- Derived from:
- ccsbBroadEn_15109
- DNA Barcode:
- AAGCGTCGCCGTGCCTCTTGTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIOK2 (55781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472502
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55781 | RIOK2 | RIO kinase 2 | NM_018343.3 | 99.2% | 95.6% | (many diffs) |
| 2 | human | 55781 | RIOK2 | RIO kinase 2 | NM_001159749.2 | 84.6% | 80.4% | (many diffs) |
| 3 | human | 55781 | RIOK2 | RIO kinase 2 | XM_017009628.1 | 65.4% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1719
- ORF length:
- 1653
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaaagtgaat gtggccaagt tgcgttacat gagccgagat gacttcaggg 121 tcttgaccgc ggttgaaatg ggcatgaaga accatgaaat tgttcccggc agtttgattg 181 cttctatagc cagccttaaa catggtggct gtaataaagt tttaagagaa ttagtgaaac 241 ataaactcat agcttgggag cgtaccaaaa ctgtccaggg ctatcggttg acaaatgcag 301 gatatgatta cctagctttg aaaacacttt cttctaggca agtagttgag tctgttggaa 361 accagatggg tgttggcaaa gaatcagata tttacattgt tgcaaatgaa gaaggacaac 421 aatttgcatt aaagcttcac agactaggaa gaacctcgtt tcgaaatttg aaaaacaaac 481 gcgattatca taaatatagg cacaatgtgt catggctata tttatctcgt ctctctgcca 541 tgaaggaatt tgcctatatg aaggcattgt atgagaggaa atttccagtt ccaaagccaa 601 ttgattacaa tcgtcatgca gtggtcatgg aactcataaa tggttatcca ctatgtcaga 661 tacaccatgt tgaagatcct gcatcagtat atgatgaagc tatggaacta attgtcaaac 721 ttgcaaatca tgggctgatt catggagatt ttaatgaatt taatctcatt ttggatgaaa 781 gtgaccatat caccatgatt gattttccac agatggtttc aacttctcat cccaatgctg 841 agtggtattt tgaacaggag aaatgcatta aagatttctt tatgaaacgt ttcagctacg 901 aaagtgagct ttttccaact tttaaggata tcagaagaga agacactctt gatgtggagt 961 ttctgccagt ggctacacaa aggaaatgca ggcagatgat gaactgcttc atcccattag 1021 gtccagatga taaaaatatt gaacaaaaag agggatctga attctcattt tcagatggag 1081 aagtggcaga aaaagcagag gtttacgggt cagaaaatga aagtgaacgg aactgtctag 1141 aagaatcaga gggctgctat tgcagatcat ctggagaccc tgaacaaata aaggaagaca 1201 gtttatcaga agagagtgct gatgcacgga gttttgaaat gactgaattc aatcaagctt 1261 tagaagaaat aaaagggcag gttgttgaaa acaactctgt aactgaaTTT TCTGAGGAGA 1321 AAAACAGAAC TGAAAATTAC AACAGGCAAG ATGGTCAGAG AGTTCAAGGA GGAGTCCCTG 1381 CTGGCTCTGA CGAGTATGAA GATGAATGCC CTCATCTAAT TGCCTTGTCG TCATTAAATA 1441 GAGAATTCAG GCCTTTCAGA GATGAAGAAA ATGTGGGAGC TATGAATCAG TATAGAACAA 1501 GAACTCTGAG TATCACTTCT TCAGGCAGTG CTGTAAGCTG TTCAACAATT CCTCCAGAAC 1561 TGGTGAAACA GAAGGTGAAA CGTCAGTTGA CAAAACAGCA AAAATCAGCT GTCAGACGTC 1621 GATTGCAGAA AGGAGAAGCA AATATATTTA CCAAGCAACG TAGGGAAAAC ATGCAAAATA 1681 TCAAATCAAG TTTGGAAGCA GCCAGCTTTT GGGGAGAATT GCCAACTTTC TTGTACAAAG 1741 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1801 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1861 ACGAAAGCGT CGCCGTGCCT CTTGTACTAC GCGTTAAGTC gacaatcaac ctctggatta 1921 caaaatttgt gaaagatt