Construct: ORF TRCN0000472514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017945.1_s317c1
- Derived from:
- ccsbBroadEn_00340
- DNA Barcode:
- TTTACCGGTTTAAAATATGTATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNTF (1270)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1270 | CNTF | ciliary neurotrophic factor | NM_000614.4 | 100% | 100% | |
| 2 | human | 386607 | ZFP91-CNTF | ZFP91-CNTF readthrough (NMD... | NR_024091.1 | 16.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 666
- ORF length:
- 600
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttcacagag cattcaccgc tgacccctca ccgtcgggac ctctgtagcc 121 gctctatctg gctagcaagg aagattcgtt cagacctgac tgctcttacg gaatcctatg 181 tgaagcatca gggcctgaac aagaacatca acctggactc tgcggatggg atgccagtgg 241 CAAGCACTGA TCAGTGGAGT GAGCTGACCG AGGCAGAGCG ACTCCAAGAG AACCTTCAAG 301 CTTATCGTAC CTTCCATGTT TTGTTGGCCA GGCTCTTAGA AGACCAGCAG GTGCATTTTA 361 CCCCAACCGA AGGTGACTTC CATCAAGCTA TACATACCCT TCTTCTCCAA GTCGCTGCCT 421 TTGCATACCA GATAGAGGAG TTAATGATAC TCCTGGAATA CAAGATCCCC CGCAATGAGG 481 CTGATGGGAT GCCTATTAAT GTTGGAGATG GTGGTCTCTT TGAGAAGAAG CTGTGGGGCC 541 TAAAGGTGCT GCAGGAGCTT TCACAGTGGA CAGTAAGGTC CATCCATGAC CTTCGTTTCA 601 TTTCTTCTCA TCAGACTGGG ATCCCAGCAC GTGGGAGCCA TTATATTGCT AACAACAAGA 661 AAATGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 721 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 781 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTTACCGGT TTAAAATATG TATTTACGCG 841 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt