Construct: ORF TRCN0000472522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003949.1_s317c1
- Derived from:
- ccsbBroadEn_11815
- DNA Barcode:
- TCCTGTGGTGATCTGAGCGCGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTLL3 (26140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472522
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100526693 | ARPC4-TTLL3 | ARPC4-TTLL3 readthrough | NM_001198793.1 | 16.1% | 16% | 1_999del;1302_1875delinsG |
| 2 | human | 26140 | TTLL3 | tubulin tyrosine ligase like 3 | NM_001366051.2 | 13% | 12.9% | 1_816del;1119_2316delinsG |
| 3 | human | 26140 | TTLL3 | tubulin tyrosine ligase like 3 | NM_001025930.5 | 11% | 10.9% | 1_1245del;1548_2745delinsG |
| 4 | mouse | 101100 | Ttll3 | tubulin tyrosine ligase-lik... | NM_133923.6 | 9.7% | 9.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 369
- ORF length:
- 303
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aggggatcgc aacatcTGGA TCGTGAAGCC AGGAGCCAAG TCCCGCGGAC 121 GAGGCATCAT GTGCATGGAC CACCTGGAGG AGATGCTGAA GCTGGTGAAC GGCAACCCCG 181 TGGTGATGAA GGACGGCAAG TGGGTGGTGC AGAAGTATAT TGAGCGGCCC CTCCTCATCT 241 TTGGCACCAA GTTTGACCTC AGACAGTGGT TCCTGGTAAC TGACTGGAAC CCACTTACCG 301 TGTGGTTCTA CCGCGACAGC TATATCCGCT TTTCCACGCA GCCCTTCTCC CTGAAGAACC 361 TGGACAAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCCTGT GGTGATCTGA GCGCGTCTAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt