Construct: ORF TRCN0000472535

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009574.1_s317c1
Derived from:
ccsbBroadEn_11532
DNA Barcode:
AGGTTCTACTCCATGTTTAAATCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDPN (10630)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472535
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10630 PDPN podoplanin NM_001006624.2 73.8% 73.4% 0_1ins126;314C>G
2 human 10630 PDPN podoplanin XM_006710295.1 73.8% 73.4% 0_1ins126;314C>G
3 human 10630 PDPN podoplanin XM_024451404.1 73.8% 73.4% 0_1ins126;314C>G
4 human 10630 PDPN podoplanin NM_001006625.2 72.6% 71.6% 0_1ins126;314C>G;350_351insGTACTC
5 human 10630 PDPN podoplanin XM_024451415.1 72.6% 71.6% 0_1ins126;314C>G;350_351insGTACTC
6 human 10630 PDPN podoplanin NM_006474.4 67.9% 67.6% 1_228del;668C>G
7 human 10630 PDPN podoplanin NM_198389.2 67% 66.3% 1_228del;668C>G;704_705insGTACTC
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
552
ORF length:
486
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg gaaggtgtca gctctgctcT TCGTTTTGGG AAGCGCGTCG CTCTGGGTCC
121TGGCAGAAGG AGCCAGCACA GGCCAGCCAG AAGATGACAC TGAGACTACA GGTTTGGAAG
181GCGGCGTTGC CATGCCAGGT GCCGAAGATG ATGTGGTGAC TCCAGGAACC AGCGAAGACC
241GCTATAAGTC TGGCTTGACA ACTCTGGTGG CAACAAGTGT CAACAGTGTA ACAGGCATTC
301GCATCGAGGA TCTGCCAACT TCAGAAAGCA CAGTCCACGC GCAAGAACAA AGTCCAAGCG
361CCACAGCCTC AAACGTGGCC ACCAGTCACT CCACGGAGAA AGTGGATGGA GACACACAGA
421CAACAGTTGA GAAAGATGGT TTGTCAACAG TGACCCTGGT TGGAATCATA GTTGGGGTCT
481TACTAGCCAT CGGCTTCATT GGTGGAATCA TCGTTGTGGT TATGCGAAAA ATGTCGGGAA
541GGTACTCGCC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
601ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
661CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGG TTCTACTCCA TGTTTAAATC
721AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)