Construct: ORF TRCN0000472546
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005532.1_s317c1
- Derived from:
- ccsbBroadEn_00413
- DNA Barcode:
- CAACACCTACATCGAGTACAAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP4B1 (1580)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472546
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | NM_001099772.2 | 100% | 100% | |
| 2 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | NM_000779.4 | 99.8% | 99.8% | 617_618insCAG |
| 3 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | XR_946559.2 | 97.2% | (many diffs) | |
| 4 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | NM_001319161.2 | 97% | 97% | 321_322ins45 |
| 5 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | XM_017000466.1 | 96.8% | 96.8% | 321_322ins45;572_573insCAG |
| 6 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | NM_001319162.2 | 68.1% | 68.1% | 0_1ins489 |
| 7 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | NM_001319163.2 | 67.9% | 67.9% | 0_1ins489;128_129insCAG |
| 8 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | NR_135003.2 | 67.2% | 1_36del;653_654ins73;1500_2101del | |
| 9 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | XM_011540832.2 | 57.8% | 57.4% | 883_884insAT;888_889ins646 |
| 10 | human | 1580 | CYP4B1 | cytochrome P450 family 4 su... | XM_011540833.1 | 54.2% | 42.7% | 0_1ins700;183_184insAT |
| 11 | mouse | 13120 | Cyp4b1 | cytochrome P450, family 4, ... | NM_007823.2 | 85.6% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1602
- ORF length:
- 1536
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcccagcttc ctctccctga gcttctcctc cttgggcctg tgggcttctg 121 ggctgatctt ggtcttaggc tttctcaagc tcatccacct gctgctgcgg aggcagacgt 181 tggctaaggc tatggacaaa ttcccagggc ctcccaccca ctggcttttt ggacatgccc 241 tcgagatcca ggagacgggg agcctggaca aagtggtgtc ctgggcccac cagttcccgt 301 atgcccaccc actctggttc ggacagttca ttggcttcct gaacatctat gagcctgact 361 atgccaaagc tgtgtacagc cgtggggacc ctaaggcccc tgatgtgtat gacttcttcc 421 tccagtggat tgggagaggc ctgctggttc ttgaggggcc caagtggttg cagcaccgca 481 agctgctcac acctggcttt cattatgatg tgctgaagcc ctatgtggcc gtgttcactg 541 agtctacacg tatcatgctg gacaagtggg aagagaaagc tcgggagggt aagtcctttg 601 acatcttctg cgatgtgggt cacatggcgc tgaacacact catgaagtgc acctttggaa 661 gaggagacac cggcctgggc cacagcaggg acagcagcta ctaccttgca gtcagcgatc 721 tcactctgtt gatgcagcag cgccttgtgt ccttccagta ccataatgac ttcatctact 781 ggctcacccc acatggccgc cgcttcctgc gggcctgcca ggtggcccat gaccatacag 841 accaggtcat cagggagcgg aaggcagccc tgcaggatga gaaggtgcgg aagaagatcc 901 agaaccggag gcacctggac ttcctggaca ttctcctggg tgcccgggat gaagatgaca 961 tcaaactgtc agatgcagac ctccgggctg aagtggacac attcatgttt gaaggccatg 1021 acaccaccac cagtggtatc tcctggtttc tctactgcat ggccctgtac cctgagcacc 1081 agcatcgttg tagagaggag gtccgcgaga tcctagggga ccaggacttc ttccagtggg 1141 atgatctggg caaaatgact tatctgacca tgtgcaTCAA GGAGAGCTTC CGCCTCTACC 1201 CACCTGTGCC CCAGGTGTAC CGCCAGCTCA GCAAGCCTGT CACCTTTGTG GATGGCCGGT 1261 CTCTACCTGC AGGAAGCCTG ATCTCTATGC ATATCTATGC CCTCCATAGG AACAGTGCTG 1321 TATGGCCCGA CCCTGAGGTC TTTGACTCTC TGCGCTTTTC CACTGAGAAT GCATCCAAAC 1381 GCCATCCCTT TGCCTTTATG CCCTTCTCTG CTGGGCCCAG GAACTGCATT GGGCAGCAGT 1441 TTGCCATGAG TGAGATGAAG GTGGTCACAG CCATGTGCTT GCTCCGCTTT GAGTTCTCTC 1501 TGGACCCCTC ACGGCTGCCC ATCAAGATGC CCCAGCTTGT CCTGCGCTCC AAGAATGGCT 1561 TTCACCTCCA CCTGAAGCCA CTGGGCCCTG GGTCTGGGAA GTACCCAACT TTCTTGTACA 1621 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1681 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1741 AGGACGACAA CACCTACATC GAGTACAAGC AACGCGTTAA GTCgacaatc aacctctgga 1801 ttacaaaatt tgtgaaagat t