Construct: ORF TRCN0000472562
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001032.1_s317c1
- Derived from:
- ccsbBroadEn_09175
- DNA Barcode:
- CCTCTGCCGCGGCGTTAATTGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF1AD (84285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472562
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84285 | EIF1AD | eukaryotic translation init... | NM_001242481.2 | 99.7% | 99.3% | 67G>A |
| 2 | human | 84285 | EIF1AD | eukaryotic translation init... | NM_001242482.2 | 99.7% | 99.3% | 67G>A |
| 3 | human | 84285 | EIF1AD | eukaryotic translation init... | NM_001242483.2 | 99.7% | 99.3% | 67G>A |
| 4 | human | 84285 | EIF1AD | eukaryotic translation init... | NM_001242484.2 | 99.7% | 99.3% | 67G>A |
| 5 | human | 84285 | EIF1AD | eukaryotic translation init... | NM_001242485.2 | 99.7% | 99.3% | 67G>A |
| 6 | human | 84285 | EIF1AD | eukaryotic translation init... | NM_001242486.2 | 99.7% | 99.3% | 67G>A |
| 7 | human | 84285 | EIF1AD | eukaryotic translation init... | NM_032325.4 | 99.7% | 99.3% | 67G>A |
| 8 | human | 84285 | EIF1AD | eukaryotic translation init... | XM_017018412.2 | 99.7% | 99.3% | 67G>A |
| 9 | mouse | 69860 | Eif1ad | eukaryotic translation init... | NM_027236.2 | 86.2% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcaggccacc aagaggaagc atgtggtgaa ggaggtgcta ggggagcaca 121 tagtgcccTC CAACCAGCAG CAGATTGTCA GGGTACTCAG GACCCCAGGG AACAATCTGC 181 ATGAGGTGGA GACAGCCCAA GGGCAGCGCT TCCTGGTGAG CATGCCCTCC AAATACCGCA 241 AGAACATCTG GATCAAGAGA GGGGACTTTC TCATTGTTGA CCCCATTGAA GAGGGAGAAA 301 AGGTGAAGGC TGAAATCTCG TTTGTGCTCT GCAAGGACCA CGTGCGCTCT CTGCAGAAGG 361 AGGGGTTTTG GCCTGAGGCC TTCTCTGAAG TGGCTGAGAA ACACAACAAC AGGAACAGAC 421 AAACTCAACC AGAACTCCCA GCTGAGCCAC AGTTATCAGG AGAGGAGTCC AGCTCAGAAG 481 ATGATTCTGA CCTGTTTGTT AACACAAACC GCAGACAGTA TCATGAGAGT GAGGAGGAGA 541 GTGAAGAGGA GGAGGCAGCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCTC TGCCGCGGCG 721 TTAATTGTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt