Transcript: Human NM_032325.4

Homo sapiens eukaryotic translation initiation factor 1A domain containing (EIF1AD), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
EIF1AD (84285)
Length:
2887
CDS:
336..833

Additional Resources:

NCBI RefSeq record:
NM_032325.4
NBCI Gene record:
EIF1AD (84285)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032325.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000229902 GGTGAGCATGCCCTCCAAATA pLKO_005 485 CDS 100% 13.200 18.480 N EIF1AD n/a
2 TRCN0000130515 GAAGGCTGAAATCTCGTTTGT pLKO.1 575 CDS 100% 4.950 6.930 N EIF1AD n/a
3 TRCN0000149930 GTGAAGGCTGAAATCTCGTTT pLKO.1 573 CDS 100% 4.950 6.930 N EIF1AD n/a
4 TRCN0000218505 CTTGCAGTTCATACCAATTTA pLKO_005 1243 3UTR 100% 15.000 12.000 N EIF1AD n/a
5 TRCN0000150002 CCTGTCTATTGGATGTGATAT pLKO.1 2625 3UTR 100% 13.200 9.240 N EIF1AD n/a
6 TRCN0000229904 TCCCAGCTGAGCCACAGTTAT pLKO_005 706 CDS 100% 13.200 9.240 N EIF1AD n/a
7 TRCN0000229903 TGAAGGCTGAAATCTCGTTTG pLKO_005 574 CDS 100% 6.000 4.200 N EIF1AD n/a
8 TRCN0000217924 GCAAGAACATCTGGATCAAGA pLKO.1 508 CDS 100% 4.950 3.465 N Eif1ad n/a
9 TRCN0000149629 GCACATATCTTCCTGTCGTAA pLKO.1 2018 3UTR 100% 4.950 3.465 N EIF1AD n/a
10 TRCN0000148885 CTGAAATCTCGTTTGTGCTCT pLKO.1 580 CDS 100% 2.640 1.848 N EIF1AD n/a
11 TRCN0000128883 GAACAGACAAACTCAACCAGA pLKO.1 683 CDS 100% 2.640 1.848 N EIF1AD n/a
12 TRCN0000229905 CTGAGACTCCAGGACCCAATT pLKO_005 830 CDS 100% 10.800 6.480 N EIF1AD n/a
13 TRCN0000147434 GAAGATGATTCTGACCTGTTT pLKO.1 747 CDS 100% 4.950 2.970 N EIF1AD n/a
14 TRCN0000430981 GCCACCATGCCTGGCTAATTT pLKO_005 2373 3UTR 100% 15.000 7.500 Y GTF2IRD2 n/a
15 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 2444 3UTR 100% 4.950 2.475 Y LOC387873 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032325.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09175 pDONR223 100% 99.7% 99.3% None 67G>A n/a
2 ccsbBroad304_09175 pLX_304 0% 99.7% 99.3% V5 67G>A n/a
3 TRCN0000472562 CCTCTGCCGCGGCGTTAATTGTTA pLX_317 86% 99.7% 99.3% V5 67G>A n/a
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