Construct: ORF TRCN0000472573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015051.1_s317c1
- Derived from:
- ccsbBroadEn_13278
- DNA Barcode:
- CGTTTGAATTCCCGACATATAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASPRV1 (151516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472573
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151516 | ASPRV1 | aspartic peptidase retrovir... | NM_152792.2 | 44.3% | 44.3% | 1_573del |
| 2 | mouse | 67855 | Asprv1 | aspartic peptidase, retrovi... | NM_026414.2 | 35.1% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg taagggctac tatctcaagg ggaagattgg caaagtgccc gtgaggttcc 121 tggtggacTC TGGGGCCCAG GTCTCTGTGG TCCACCCAAA CTTGTGGGAG GAGGTCACTG 181 ATGGCGATCT GGACACCCTG CAGCCCTTTG AGAATGTGGT AAAGGTGGCC AATGGTGCTG 241 AAATGAAGAT CCTGGGTGTC TGGGATACAG CGGTGTCCCT AGGCAAGCTG AAGCTGAAGG 301 CACAGTTCCT AGTGGCCAAT GCGAGTGCCG AGGAAGCCAT CATTGGCACT GATGTGCTCC 361 AGGACCACAA TGCTATCCTG GACTTTGAGC ACCGCACATG CACCCTGAAA GGGAAGAAGT 421 TTCGCCTTCT GCCTGTGGGA GGGTCCCTGG AAGATGAGTT TGACCTGGAG CTCATAGAGG 481 AGGACCCCTC CTCAGAAGAA GGGCGGCAGG AGCTATCCCA CTGCCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGACGT TTGAATTCCC GACATATAAT TACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t