Construct: ORF TRCN0000472580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004288.1_s317c1
- Derived from:
- ccsbBroadEn_11960
- DNA Barcode:
- TAATCCGCTGGCCGCCTCCCCCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAGPA (51172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51172 | NAGPA | N-acetylglucosamine-1-phosp... | XR_001751912.2 | 64.6% | 1_26del;359A>G;954_1433del | |
| 2 | human | 51172 | NAGPA | N-acetylglucosamine-1-phosp... | XM_011522517.3 | 63.3% | 63% | (many diffs) |
| 3 | human | 51172 | NAGPA | N-acetylglucosamine-1-phosp... | NM_016256.4 | 59.8% | 59.6% | (many diffs) |
| 4 | human | 51172 | NAGPA | N-acetylglucosamine-1-phosp... | XR_001751911.2 | 41.7% | 1_26del;359A>G;954_2219del | |
| 5 | human | 51172 | NAGPA | N-acetylglucosamine-1-phosp... | XR_001751908.2 | 40.9% | (many diffs) | |
| 6 | human | 51172 | NAGPA | N-acetylglucosamine-1-phosp... | XR_001751909.2 | 40.6% | (many diffs) | |
| 7 | human | 51172 | NAGPA | N-acetylglucosamine-1-phosp... | XR_001751910.2 | 40.2% | 1_26del;359A>G;954_2303del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacctccacg ggtcgctggc ttctcctccg gcttgcacta ttcggcttcc 121 tctgggaagc gtccggcggc ctcgactcgg gggcctcccg cgacgacgac ttgctactgc 181 cctatccacg cgcgcgcgcg cgcctccccc gggactgcac acgggtgcgc gccggcaacc 241 gcgagcacga gagttggcct ccgcctcccg cgactcccgg cgccggcggt ctggccgtgc 301 gcaccttcgt gtcgcacttc agggaccgcg cggtggccgg ccacctgacg cgggccgttg 361 agcccctgcg caccttctcg gtgctggagc ccggtgggcc cggcggctgc gcggcgagac 421 gacgcgccac cgtggaggag acggcgcggg cggccgactg ccgtgtcgcc cagaacggcg 481 gcttcttccg catgaactcg ggcgagtgcc tggggaacgt ggtgagcgac gagcggcggg 541 tgagcagctc cggggggctg cagaacgcgc agttcgggat ccgccgcgac gggaccctgg 601 tcaccgggta cctgtcTGAG GAGGAGGTGC TGGACACTGA GAACCCATTT GTGCAGCTGC 661 TGAGTGGGGT CGTGTGGCTG ATTCGTAATG GAAGCATCTA CATCAACGAG AGCCAAGCCA 721 CAGAGTGTGA CGAGACACAG GAGACAGGTT CCTTTAGCAA ATTTGTGAAT GTGATATCAG 781 CCAGGACGGC CATTGGCCAC GACCGGAAAG GGCAGCTGGT GCTCTTTCAT GCAGACGGCC 841 AAACGGAGCA GCGTGGCATC AACCTGTGGG AAATGGCGGA GTTCCTGCTG AAACAGGACG 901 TGGTCAACGC CATCAACCTG GATGGGGGTG GCTCTGCCAC CTTTGTGCTC AACGGGACCT 961 TGGCCAGTTA CCCGTCAGAT CACTGGCAGG CGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1021 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1081 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1141 ATCCGCTGGC CGCCTCCCCC TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1201 ttgtgaaaga tt