Construct: ORF TRCN0000472614
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014782.1_s317c1
- Derived from:
- ccsbBroadEn_08121
- DNA Barcode:
- ACACTGTTAGCTCGTCCACATCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OLA1 (29789)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472614
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29789 | OLA1 | Obg like ATPase 1 | NM_013341.5 | 99.8% | 99.4% | 15G>T;1173A>C |
| 2 | human | 29789 | OLA1 | Obg like ATPase 1 | NM_001328688.2 | 94.5% | 93.9% | 15G>T;727_728ins63;1110A>C |
| 3 | human | 29789 | OLA1 | Obg like ATPase 1 | NM_001011708.3 | 60% | 59.8% | 0_1ins474;699A>C |
| 4 | mouse | 67059 | Ola1 | Obg-like ATPase 1 | NM_025942.2 | 93.3% | 98.9% | (many diffs) |
| 5 | mouse | 67059 | Ola1 | Obg-like ATPase 1 | NM_030091.1 | 59.7% | 62.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1254
- ORF length:
- 1188
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ccctaaaaat ggaggtgatg gaattaaacc acccccaatc attggaagat 121 ttggaacctc actgaaaatt ggtattgttg gattgccaaa tgttgggaaa tctactttct 181 tcaatgtgtt aaccaatagt caggcttcag cagaaaactt cccgttctgc actattgatc 241 ctaatgagag cagagtacct gtgccagatg aaaggtttga ctttctttgt caataccaca 301 aaccagcaag caaaattcct gcctttctaa atgtggtgga tattgctggc cttgtgaaag 361 gagctcacaa tgggcagggc ctggggaatg cttttttatc tcatattagt gcctgtgatg 421 gcatctttca tctaacacgt gcttttgaag atgatgatat cacgcacgtt gaaggaagtg 481 tagatcctat tcgagatata gaaataatac atgaagagct tcagcttaaa gatgaggaaa 541 tgattgggcc cattatagat aaactagaaa aggtggctgt gagaggagga gataaaaaac 601 taaaacctga atatgatata atgtgcaaag taaaatcctg ggttatagat caaaagaaac 661 ctgttcgctt ctatcatgat tggaatgaca aagagattga agtgttgaat aaacacttat 721 ttttgacttc aaaaccaatg gtctacttgg ttaatctttc tgaaaaagac tacattagaa 781 agaaaaacaa atggttgata aaaaTTAAAG AGTGGGTGGA CAAGTATGAC CCAGGTGCTT 841 TGGTCATTCC TTTTAGTGGG GCCTTGGAAC TCAAGTTGCA AGAATTGAGT GCTGAGGAGA 901 GACAGAAGTA TCTGGAAGCG AACATGACAC AAAGTGCTTT GCCAAAGATC ATTAAGGCTG 961 GGTTTGCAGC ACTCCAACTA GAATACTTTT TCACTGCAGG CCCAGATGAA GTGCGTGCAT 1021 GGACCATCAG GAAAGGGACT AAGGCTCCTC AGGCTGCAGG AAAGATTCAC ACAGATTTTG 1081 AAAAGGGATT CATTATGGCT GAAGTAATGA AATACGAAGA TTTTAAAGAG GAAGGTTCTG 1141 AAAATGCAGT CAAGGCTGCT GGAAAGTACA GACAACAAGG CAGAAATTAT ATTGTTGAAG 1201 ATGGAGATAT TATCTTCTTC AAATTTAACA CACCTCACCA ACCGAAGAAG AAATACCCAA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATCTTGTGG AAAGGACGAA CACTGTTAGC TCGTCCACAT CGTACGCGTT AAGTCgacaa 1441 tcaacctctg gattacaaaa tttgtgaaag att