Construct: ORF TRCN0000472629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008506.1_s317c1
- Derived from:
- ccsbBroadEn_14332
- DNA Barcode:
- ACGCGTGTGCACGTCTCTGCCCGA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEACAM21 (90273)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90273 | CEACAM21 | CEA cell adhesion molecule 21 | NM_033543.6 | 50.8% | 49% | 423_424ins83;488T>C;490_876del |
| 2 | human | 90273 | CEACAM21 | CEA cell adhesion molecule 21 | XM_005278397.4 | 50.8% | 49% | 423_424ins83;488T>C;490_876del |
| 3 | human | 90273 | CEACAM21 | CEA cell adhesion molecule 21 | NM_001098506.4 | 50.7% | 48.8% | 423_424ins83;488T>C;490_879del |
| 4 | human | 90273 | CEACAM21 | CEA cell adhesion molecule 21 | XM_005278396.4 | 50.7% | 48.8% | 423_424ins83;488T>C;490_879del |
| 5 | human | 90273 | CEACAM21 | CEA cell adhesion molecule 21 | XM_017027432.2 | 46.6% | 40% | (many diffs) |
| 6 | human | 90273 | CEACAM21 | CEA cell adhesion molecule 21 | XM_017027430.1 | 46.5% | 39.8% | (many diffs) |
| 7 | human | 90273 | CEACAM21 | CEA cell adhesion molecule 21 | XM_017027431.1 | 46.5% | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcccccctca gcttgtcccc acagagaatg catcccctgg caggggctct 121 tgctcacagc ctcactttta actttctgga acgcacccac cactgcctgg ctctttattg 181 catcagcgcc ctttGAAGTT GCTGAAGGGG AGAATGTTCA TCTCTCTGTG GTTTATCTGC 241 CCGAGAATCT TTACAGCTAT GGCTGGTACA AAGGGAAAAC GGTGGAGCCC AACCAGCTAA 301 TCGCAGCATA TGTAATAGAC ACTCACGTTA GGACTCCAGG GCCTGCATAC AGCGGTCGAG 361 AGACAATATC ACCCAGTGGA GATCTGCATT TCCAGAACGT CACCCTAGAG GACACGGGAT 421 ACTACAACCT ACAAGTCACA TACAGAAATT CTCAGATTGA ACAGGCATCT CACCATCTCC 481 GTGTATACGG AGAATGTTCT AAATTTGACT CTGAGATCTC TGAGGATGCA GCATGGCCCC 541 AAGACACTTT CTGTTGGTCA CTCTATCCAC AGAGTCAGTG GCTCAGCCCT CCATCCAAGC 601 CAGCAGCACC ACAGTCACAG AGAAGGGCTC CGTGGCCTGC CCAACTTTCT TGTACAAAGT 661 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 721 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 781 CGAACGCGTG TGCACGTCTC TGCCCGAACG CGTTAAGTCg acaatcaacc tctggattac 841 aaaatttgtg aaagatt