Construct: ORF TRCN0000472645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014674.1_s317c1
- Derived from:
- ccsbBroadEn_06223
- DNA Barcode:
- CTCGCCCATTAAGATGCGGACGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRZB (2487)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2487 | FRZB | frizzled related protein | NM_001463.4 | 99.8% | 99.6% | 975C>A |
| 2 | mouse | 20378 | Frzb | frizzled-related protein | NM_011356.4 | 89.1% | 91.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ctgcggcagc ccgggaggga tgctgctgct gcgggccggg ctgcttgccc 121 tggctgctct ctgcctgctc cgggtgcccg gggctcgggc tgcagcctgt gagcccgtcc 181 gcatccccct gtgcaagtcc ctgccctgga acatgactaa gatgcccaac cacctgcacc 241 acagcactca ggccaacgcc atcctggcca tcgagcagtt cgaaggtctg ctgggcaccc 301 actgcagccc cgatctgctc ttcttcctct gtgccatgta cgcgcccatc tgcaccattg 361 acttccagca cgagcccatc aagccctgta agtctgtgtg cgagcgggcc cggcagggct 421 gtgagcccat actcatcaag taccgccact cgtggccgga gaacctggcc tgcgaggagc 481 tgccagtgta cgacaggggc gtgtgcatct ctcccgaggc catcgttact gcggacggag 541 ctgattttcc tatggattct agtaacggaa actgtagagg ggcaagcagt gaacgctgta 601 aatgtaagcc TATTAGAGCT ACACAGAAGA CCTATTTCCG GAACAATTAC AACTATGTCA 661 TTCGGGCTAA AGTTAAAGAG ATAAAGACTA AGTGCCATGA TGTGACTGCA GTAGTGGAGG 721 TGAAGGAGAT TCTAAAGTCC TCTCTGGTAA ACATTCCACG GGACACTGTC AACCTCTATA 781 CCAGCTCTGG CTGCCTCTGC CCTCCACTTA ATGTTAATGA GGAATATATC ATCATGGGCT 841 ATGAAGATGA GGAACGTTCC AGATTACTCT TGGTGGAAGG CTCTATAGCT GAGAAGTGGA 901 AGGATCGACT CGGTAAAAAA GTTAAGCGCT GGGATATGAA GCTTCGTCAT CTTGGACTCA 961 GTAAAAGTGA TTCTAGCAAT AGTGATTCCA CTCAGAGTCA GAAGTCTGGC AGGAACTCGA 1021 ACCCCCGGCA AGCACGCAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTCG CCCATTAAGA 1201 TGCGGACGTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt