Construct: ORF TRCN0000472677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017924.1_s317c1
- Derived from:
- ccsbBroadEn_02360
- DNA Barcode:
- TGGCCTCGTATAACACATTGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPA33 (10223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10223 | GPA33 | glycoprotein A33 | NM_005814.3 | 100% | 100% | |
| 2 | human | 10223 | GPA33 | glycoprotein A33 | XM_017000005.1 | 66.1% | 66.1% | 0_1ins324 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1023
- ORF length:
- 957
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggggaagatg tggcctgtgt tgtggacact ctgtgcagtc agggtgaccg 121 tcgatgccat ctctgtggaa actccgcagg acgttcttcg ggcttcgcag ggaaagagtg 181 tcaccctgcc ctgcacctac cacacttcca cctccagtcg agagggactt attcaatggg 241 ataagctcct cctcactcat acggaaaggg tggtcatctg gccgttttca aacaaaaact 301 acatccatgg tgagctttat aagaatcgcg tcagcatatc caacaatgct gagcagtccg 361 atgcctccat caccattgat cagctgacca tggctgacaa cggcacctac gagtgttctg 421 tctcgctgat gtcagacctg gagggcaaca ccaagtcacg tgtccgcctg ttggtcctcg 481 tgccaccctc caaaccagaa tgcggcatcg agggagagac cataattggg aacaacatcc 541 agctgacctg ccaatcaaag gagggctcac caacccctca gtacagctgg aagaggtaca 601 acatcctgaa tcaggagcag cccctggccc agccagcctc aggtcagcct gtctcccTGA 661 AGAATATCTC CACAGACACA TCGGGTTACT ACATCTGTAC CTCCAGCAAT GAGGAGGGGA 721 CGCAGTTCTG CAACATCACG GTGGCCGTCA GATCTCCCTC CATGAACGTG GCCCTGTATG 781 TGGGCATCGC GGTGGGCGTG GTTGCAGCCC TCATTATCAT TGGCATCATC ATCTACTGCT 841 GCTGCTGCCG AGGGAAGGAC GACAACACTG AAGACAAGGA GGATGCAAGG CCGAACCGGG 901 AAGCCTATGA GGAGCCACCA GAGCAGCTAA GAGAACTTTC CAGAGAGAGG GAGGAGGAGG 961 ATGACTACAG GCAAGAAGAG CAGAGGAGCA CTGGGCGTGA ATCCCCGGAC CACCTCGACC 1021 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGATG GCCTCGTATA ACACATTGGC ACACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt