Construct: ORF TRCN0000472706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015057.1_s317c1
- Derived from:
- ccsbBroadEn_10943
- DNA Barcode:
- TTTTCAGCACCTCTGTGAGTCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAMC1 (3915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3915 | LAMC1 | laminin subunit gamma 1 | NM_002293.4 | 2.3% | .6% | 1_3706del;3821_4827del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 314
- ORF end:
- 428
- ORF length:
- 114
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCATGAACA AGCGAAGAAC ATCTCACAGG ATCTGGAAAA ACAAGCTGCC 361 CGAGTACATG AGGAGGCCAA AAGGGCCGGT GACAAAGCTG TGGAGATCTA TGCCAGCGTG 421 GCTCAGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 481 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 541 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTTTCAG CACCTCTGTG AGTCGCCACG 601 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt