Construct: ORF TRCN0000472734
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003518.1_s317c1
- Derived from:
- ccsbBroadEn_13410
- DNA Barcode:
- TCGGTCACTAGTTACTCTTGAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC83 (220047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472734
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220047 | CCDC83 | coiled-coil domain containi... | NM_001286159.1 | 99.9% | 99.7% | 680T>C |
| 2 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_017017354.1 | 99.6% | 99.5% | 680T>C;872_873insAGA |
| 3 | human | 220047 | CCDC83 | coiled-coil domain containi... | NM_173556.4 | 92.9% | 92.7% | 680T>C;794_886del |
| 4 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_011544839.2 | 92.9% | 92.7% | 680T>C;794_886del |
| 5 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_011544840.2 | 92.9% | 92.7% | 680T>C;794_886del |
| 6 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_011544841.1 | 92.9% | 92.7% | 680T>C;794_886del |
| 7 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_011544842.1 | 92.7% | 92.5% | 680T>C;794_886del;965_966insAGA |
| 8 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_017017355.1 | 86.1% | 85.9% | 341_342ins168;512T>C;704_705insAGA |
| 9 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_011544843.1 | 80.3% | 80.1% | 341_342ins168;512T>C;626_718del |
| 10 | human | 220047 | CCDC83 | coiled-coil domain containi... | XM_011544844.2 | 70.8% | 70.7% | 0_1ins294;386T>C;500_592del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1305
- ORF length:
- 1239
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaactcaggg aaagcaaata aaaaggatac acatgacggg ccaccaaaag 121 aaattaaact gcctaccagt gaagcacttc tagactatca atgtcaaata aaggaagatg 181 ccgtggagca attcatgttt caaataaaga cacttaggaa aaagaaccaa aaatatcatg 241 aaagaaatag ccgcttaaaa gaagaacaga tttggcacat acggcatcta ctaaaggaac 301 tgagtgaaga gaaggcagag ggattgccag ttgtaacaag agaggatgtt gaagaagcga 361 tgaaggaaaa atggaagttt gaaagagacc aggaaaaaaa cttgagagat atgcgcatgc 421 aaataagtaa tgctgagaaa ctatttcttg agaaactcag tgaaaaggaa tattgggagg 481 agtacaagaa tgtagggagt gaacgacatg ctaaactcat tacctcctta caaaatgaca 541 tcaacacagt taaagagaat gcagagaaaa tgtcagaaca ctataaaatc actctggaag 601 atactagaaa gaaaataatc aaggaaactt tgttgcaact ggaccaaaag aaggaatggg 661 ccacacagaa tgctgtaaag ctcattgaca agggcagtta tctagagatc tgggagaatg 721 actggctcaa aaaagaggtt gcaactcaca ggaaggaagt tGAAGAATTA AAAAATGCTA 781 TTCATGAACT GGAAGCAGAA AATTTGGTGC TTATTGATCA ACTATCCAAC TGTAGACTTG 841 TGGATCTCAA GATACCCAGG CGACTATATC TTACCCAAGC TGCTGGACTA GAAGTGCCAC 901 CTGAAGAAAT GTCTTTGGAA TTGCCAGAAA CACATATAGA AGAGAAGTCA GAATTGCAAC 961 CCACAGAAGT AGAAAGTAGA GACTTGATGT CCTCATCAGA TGAGAGCACT ATCTTACATC 1021 TTAGTCATGA AAATAGCATC GAAGATCTCC AGTATGTGAA GATAGATAAA GAGGAAAACT 1081 CAGGCACAGA GTTTGGGGAC ACTGATATGA AGTACTTACT ATATGAGGAT GAGAAGGATT 1141 TCAAGGATTA TGTAAACTTG GGCCCCCTGG GAGTGAAGCT TATGAGTGTG GAGAGCAAGA 1201 AAATGCCCAT TCATTTTCAA GAGAAGGAAA TTCCAGTCAA ACTCTATAAA GATGTCAGGA 1261 GCCCAGAAAG CCACATCACA TATAAGATGA TGAAGTCTTT TCTCTACCCA ACTTTCTTGT 1321 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1381 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1441 GAAAGGACGA TCGGTCACTA GTTACTCTTG AAGTACGCGT TAAGTCgaca atcaacctct 1501 ggattacaaa atttgtgaaa gatt