Construct: ORF TRCN0000472751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016700.1_s317c1
- Derived from:
- ccsbBroadEn_06009
- DNA Barcode:
- TACTGCAGCCGATCCTTATAGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLC (1178)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1178 | CLC | Charcot-Leyden crystal gale... | NM_001828.6 | 99.5% | 99.2% | 83C>T;240T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 519
- ORF length:
- 426
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGTCCCT GCTACCCGTG CCATACACAG 121 AGGCTGCCTC TTTGTCTACT GGTTCTACTG TGACAATCAA AGGGCGACCA CTTGTCTGTT 181 TCTTGAATGA ACCATATCTG CAGGTGGATT TCCACACTGA GATGAAGGAG GAATCAGACA 241 TTGTCTTCCA TTTCCAAGTG TGCTTTGGTC GTCGTGTGGT CATGAACAGC CGTGAGTATG 301 GGGCCTGGAA GCAGCAGGTG GAATCCAAGA ACATGCCCTT TCAGGATGGC CAAGAATTTG 361 AACTGAGCAT CTCAGTGCTG CCAGATAAGT ACCAGGTAAT GGTCAATGGC CAATCCTCTT 421 ACACCTTTGA CCATAGAATC AAGCCTGAGG CTGTGAAGAT GGTGCAAGTG TGGAGAGATA 481 TCTCCCTGAC CAAATTTAAT GTCAGCTATT TAAAGAGATT GCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGATACTGC AGCCGATCCT TATAGGAAAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt