Construct: ORF TRCN0000472770
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004984.1_s317c1
- Derived from:
- ccsbBroadEn_09066
- DNA Barcode:
- TCAACCAGCCTTGATACGTATAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TMX1 (81542)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472770
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81542 | TMX1 | thioredoxin related transme... | NM_030755.5 | 99.2% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gccctccggg agtcttgcag ttcccctggc agtcatggtg ccgttgcttt 121 ggggtgctcc ctggacgcac gggcggcgga gcaacgttcg cgtcatcacg gacgagaact 181 ggagagaact gctggaagga gactggatga tagaatttta tgccccgtgg tgccctgctt 241 gtcaaaatct tcaaccggaa tgggaaagtt ttgctgaatg gggagaagat cttgaggtta 301 atattgcgaa agtagatgtc acagagcagc caggactgag tggacggttt atcataaatg 361 ctcttcctac tatttatcat tgtaaagatg gtgaatttag gcgctatcag ggtccaagga 421 ctaagaagga cttcataaac tttataagtg ataaagagtg gaagagtatt gagcccgttt 481 catcatggtt tggtccaggt tctgttctga tgagtagtat gtcagcactc tttcagctat 541 ctatgtggat caggacgtgc cataactact ttattgaaga ccttggattg ccagtgtggg 601 gatcatatac tgtttttgct ttagcaactc tgttttccgg actgttatta ggactctgta 661 tgatatttgt ggcagattgc ctttgtcctt caaaaaggcg cagaccacag ccatacccat 721 acccttcaaa aaaattatta tcagaatctg cacaaccttt gaaaaaagtg gaggaggaac 781 aAGAGGGCGG ATGAAGAAGA TGTTTCAGAA GAAGAAGCTG AAAGTAAAGA AGGAACAAAC 841 AAAGACTTTC CACAGAATGC CATAAGACAA CGCTCTCTGG GTCCATCATT GGCCACAGAT 901 AAATCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT 961 CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCTA ACTTGAAAGT ATTTCGATTT 1021 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCAACCAGC CTTGATACGT ATAATACGCG 1081 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt