Construct: ORF TRCN0000472776
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001896.1_s317c1
- Derived from:
- ccsbBroadEn_00318
- DNA Barcode:
- GCTCACACTCGTGAGCATTCACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP2M1 (1173)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472776
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1173 | AP2M1 | adaptor related protein com... | NM_004068.4 | 100% | 100% | |
| 2 | human | 1173 | AP2M1 | adaptor related protein com... | NM_001025205.1 | 99.5% | 99.5% | 419_420insTCAGCA |
| 3 | human | 1173 | AP2M1 | adaptor related protein com... | NM_001311198.1 | 94.5% | 94.5% | 74_148del |
| 4 | mouse | 11773 | Ap2m1 | adaptor-related protein com... | NM_009679.3 | 92.6% | 100% | (many diffs) |
| 5 | mouse | 11773 | Ap2m1 | adaptor-related protein com... | NM_001302970.1 | 92.1% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1371
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tggaggctta ttcatctata atcacaaggg ggaggtgctc atctcccgag 121 tctaccgaga tgacatcggg aggaacgcag tggatgcctt tcgggtcaat gttatccatg 181 cccggcagca ggtgcgcagc cccgtcacca acattgctcg caccagcttc ttccacgtta 241 agcggtccaa catttggctg gcagcagtca ccaagcagaa tgtcaacgct gccatggtct 301 tcgaattcct ctataagatg tgtgacgtga tggctgccta ctttggcaag atcagcgagg 361 aaaacatcaa gaacaatttt gtgctcatat atgagctgct ggatgagatt ctagactttg 421 gctacccaca gaattccgag acaggcgcgc tgaaaacctt catcacgcag cagggcatca 481 agagtcagca tcagacaaaa gaagagcagt cacagatcac cagccaggta actgggcaga 541 ttggctggcg gcgagagggt atcaagtatc gtcggaatga gctcttcctg gatgtgctgg 601 agagtgtgaa cctgctcatg tccccacaag ggcaggtgct gagtgcccat gtgtcgggcc 661 gggtggtgat gaagagctac ctgagtggca tgcctgaatg caagtttggg atgaatgaca 721 agattgttat tgaaaagcag ggcaaaggca cagctgatga aacaagcaag agcgggaagc 781 aatcaattgc cattgatgac tgcaCCTTCC ACCAGTGTGT GCGACTCAGC AAGTTTGACT 841 CTGAACGCAG CATCAGCTTT ATCCCGCCAG ATGGAGAGTT TGAGCTTATG AGGTATCGCA 901 CAACCAAGGA CATCATCCTT CCCTTCCGGG TGATCCCGCT AGTGCGAGAA GTGGGACGCA 961 CCAAACTGGA GGTCAAGGTG GTCATCAAGT CCAACTTTAA ACCCTCACTG CTGGCTCAGA 1021 AGATCGAGGT GAGGATCCCA ACCCCACTGA ACACAAGCGG GGTGCAGGTG ATCTGCATGA 1081 AGGGGAAGGC CAAGTACAAG GCCAGCGAGA ATGCCATCGT GTGGAAGATC AAGCGCATGG 1141 CAGGCATGAA GGAATCGCAG ATCAGCGCAG AGATTGAGCT TCTGCCTACC AACGACAAGA 1201 AGAAATGGGC TCGACCCCCC ATTTCCATGA ACTTTGAGGT GCCATTCGCG CCCTCTGGCC 1261 TCAAGGTGCG CTACTTGAAG GTGTTTGAAC CGAAGCTGAA CTACAGCGAC CATGATGTCA 1321 TCAAATGGGT GCGCTACATT GGCCGCAGTG GCATTTATGA AACTCGCTGC TACCCAACTT 1381 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1441 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1501 CTTGTGGAAA GGACGAGCTC ACACTCGTGA GCATTCACCT ACGCGTTAAG TCgacaatca 1561 acctctggat tacaaaattt gtgaaagatt