Construct: ORF TRCN0000472787
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001847.2_s317c1
- Derived from:
- ccsbBroadEn_14756
- DNA Barcode:
- CGCCGTGTAGCGCACTTGAAAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHKG2 (5261)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472787
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5261 | PHKG2 | phosphorylase kinase cataly... | NM_000294.3 | 100% | 100% | |
| 2 | human | 5261 | PHKG2 | phosphorylase kinase cataly... | NM_001172432.1 | 90.5% | 88.7% | (many diffs) |
| 3 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | NM_026888.3 | 89.5% | 93.5% | (many diffs) |
| 4 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508167.3 | 89.5% | 93.5% | (many diffs) |
| 5 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_011241891.1 | 89.5% | 93.5% | (many diffs) |
| 6 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508168.2 | 67.5% | 69.4% | (many diffs) |
| 7 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508169.3 | 67.5% | 69.4% | (many diffs) |
| 8 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508170.2 | 66.6% | 69.9% | (many diffs) |
| 9 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508171.2 | 66.6% | 69.9% | (many diffs) |
| 10 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_006508172.3 | 64.9% | 68.2% | (many diffs) |
| 11 | mouse | 68961 | Phkg2 | phosphorylase kinase, gamma... | XM_017312279.1 | 64.9% | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gctggacgtg gggccggagg atgagctgcc cgactgggcc gccgccaaag 121 agttttacca gaagtacgac cctaaggacg tcatcggcag aggagtgagc tctgtggtcc 181 gccgttgtgt tcatcgagct actggccacg agtttgcggt gaagattatg gaagtgacag 241 ctgagcggct gagtcctgag cagctggagg aggtgcggga agccacacgg cgagagacac 301 acatccttcg ccaggtcgcc ggccaccccc acatcatcac cctcatcgat tcctacgagt 361 cttctagctt catgttcctg gtgtttgacc tgatgcggaa gggagagctg tttgactatc 421 tcacagagaa ggtggccctc tctgaaaagg aaaccaggtc catcatgcgg tctctgctgg 481 aagcagtgag ctttctccat gccaacaaca ttgtgcatcg agatctgaag cccgagaata 541 ttctcctaga tgacaatatg cagatccgac tttcagattt cgggttctcc tgccacttgg 601 aacctggcga gaagcttcga gagttgtgtg ggaccccagg gtatctagcg ccagagatcc 661 ttaaatgctc catggatgaa acccacccag gctatggcaa ggaggtcgac ctctgggcct 721 gtggggtgat cttgttcaca ctcctggctg gctcgccacc cttctggcac cggcggcaga 781 tcctgatgtt acgcatgatc atggagggcc agtaccagtt cagttccccc gagtgggatg 841 accgttccag cactgtcaaa gacctgatct ccaggctgct gcaggtggat ccTGAGGCAC 901 GCCTGACAGC TGAGCAGGCC CTACAGCACC CCTTCTTTGA GCGTTGTGAA GGCAGCCAAC 961 CCTGGAACCT CACCCCCCGC CAGCGGTTCC GGGTGGCAGT GTGGACAGTG CTGGCTGCTG 1021 GACGAGTGGC CCTAAGCACC CATCGTGTAC GGCCACTGAC CAAGAATGCA CTGTTGAGGG 1081 ACCCTTATGC GCTGCGGTCA GTGCGGCACC TCATCGACAA CTGTGCCTTC CGGCTCTACG 1141 GGCACTGGGT AAAGAAAGGG GAGCAGCAGA ACCGGGCGGC TCTCTTTCAG CACCGGCCCC 1201 CTGGGCCTTT TCCCATCATG GGCCCTGAAG AGGAGGGAGA CTCTGCTGCT ATAACTGAGG 1261 ATGAGGCCGT GCTTGTGCTG GGCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTACT 1381 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG CCGTGTAGCG 1441 CACTTGAAAA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 1501 tt