Construct: ORF TRCN0000472808
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010070.1_s317c1
- Derived from:
- ccsbBroadEn_00628
- DNA Barcode:
- ATGAGAGGAAAGAGCTTGCGGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GDNF (2668)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472808
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2668 | GDNF | glial cell derived neurotro... | NM_000514.4 | 100% | 100% | |
| 2 | human | 2668 | GDNF | glial cell derived neurotro... | NM_001190468.1 | 92.5% | 92.5% | 1_51del |
| 3 | human | 2668 | GDNF | glial cell derived neurotro... | NM_199231.2 | 87.6% | 87.2% | 73_74ins78 |
| 4 | human | 2668 | GDNF | glial cell derived neurotro... | XM_017009337.2 | 87.6% | 87.2% | 73_74ins78 |
| 5 | human | 2668 | GDNF | glial cell derived neurotro... | NM_001190469.1 | 81.1% | 80.7% | 1_51del;124_125ins78 |
| 6 | human | 2668 | GDNF | glial cell derived neurotro... | NM_001278098.1 | 75.3% | 75.3% | 0_1ins156 |
| 7 | human | 2668 | GDNF | glial cell derived neurotro... | XM_011514030.3 | 75.3% | 75.3% | 0_1ins156 |
| 8 | mouse | 14573 | Gdnf | glial cell line derived neu... | NM_001301357.1 | 89.7% | 92.8% | (many diffs) |
| 9 | mouse | 14573 | Gdnf | glial cell line derived neu... | NM_001301333.1 | 81.9% | 84.8% | (many diffs) |
| 10 | mouse | 14573 | Gdnf | glial cell line derived neu... | NM_010275.3 | 78.8% | 81.6% | (many diffs) |
| 11 | mouse | 14573 | Gdnf | glial cell line derived neu... | NM_001301332.1 | 69.1% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gttatgggat gtcgtggctg tctgcctggt gctgctccac accgcgtccg 121 ccttcccgct gcccgccggt aagaggcctc ccgaggcgcc cgccgaagac cgctccctcg 181 gccgccgccg cgcgcccttc gcgctgagca gtgactcaaa tatgccagag gattatcctg 241 atcagttcGA TGATGTCATG GATTTTATTC AAGCCACCAT TAAAAGACTG AAAAGGTCAC 301 CAGATAAACA AATGGCAGTG CTTCCTAGAA GAGAGCGGAA TCGGCAGGCT GCAGCTGCCA 361 ACCCAGAGAA TTCCAGAGGA AAAGGTCGGA GAGGCCAGAG GGGCAAAAAC CGGGGTTGTG 421 TCTTAACTGC AATACATTTA AATGTCACTG ACTTGGGTCT GGGCTATGAA ACCAAGGAGG 481 AACTGATTTT TAGGTACTGC AGCGGCTCTT GCGATGCAGC TGAGACAACG TACGACAAAA 541 TATTGAAAAA CTTATCCAGA AATAGAAGGC TGGTGAGTGA CAAAGTAGGG CAGGCATGTT 601 GCAGACCCAT CGCCTTTGAT GATGACCTGT CGTTTTTAGA TGATAACCTG GTTTACCATA 661 TTCTAAGAAA GCATTCCGCT AAAAGGTGTG GATGTATCTG CCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGAATGAGA GGAAAGAGCT TGCGGTAGAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt