Construct: ORF TRCN0000472851
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008100.1_s317c1
- Derived from:
- ccsbBroadEn_02977
- DNA Barcode:
- TGAATTCGCAGAGGGAAGGGCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC22A (26984)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472851
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26984 | SEC22A | SEC22 homolog A, vesicle tr... | NM_012430.5 | 100% | 100% | |
| 2 | human | 26984 | SEC22A | SEC22 homolog A, vesicle tr... | XM_011512673.3 | 100% | 100% | |
| 3 | human | 26984 | SEC22A | SEC22 homolog A, vesicle tr... | XM_011512675.3 | 100% | 100% | |
| 4 | human | 26984 | SEC22A | SEC22 homolog A, vesicle tr... | XM_011512676.3 | 100% | 100% | |
| 5 | mouse | 317717 | Sec22a | SEC22 homolog A, vesicle tr... | NM_133704.4 | 93.3% | 96.7% | (many diffs) |
| 6 | mouse | 317717 | Sec22a | SEC22 homolog A, vesicle tr... | XM_006522235.2 | 88.7% | 91.9% | (many diffs) |
| 7 | mouse | 317717 | Sec22a | SEC22 homolog A, vesicle tr... | XM_006522234.3 | 86.6% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 987
- ORF length:
- 921
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tatgatttta tctgcctcag tcattcgtgt cagagatgga ctgccacttt 121 ctgcttctac tgattatgaa caaagcacag gaatgcagga gtgcagaaag tattttaaaa 181 tgctttcgag gaaacttgct caacttcctg atagatgtac actgaaaact ggacattata 241 acattaattt tattagctct ctgggagtga gctacatgat gttgtgcact gaaaattacc 301 caaatgttct cgccttctct ttcctggatg agcttcagaa ggagttcatt actacttata 361 acatgatgaa gacaaatact gctgtcagac catactgttt cattgaattt gataacttca 421 ttcagaggac caagcagcga tataataatc ccaggtctct ttcaacaaag ataaatcttt 481 ctgacatgca gacggaaatc aagctgaggc ctccttatca aatttccatg tgcgaactgg 541 ggtcagccaa tggagtcaca tcagcatttt ctgttgactg taaaggtgct ggtaagattt 601 cttctgctca ccagcgactg gaaccagcaa ctctgtcagg gattgtagga tttatcctTA 661 GTCTTTTATG TGGAGCTCTG AATTTAATTC GAGGCTTTCA TGCTATAGAA AGTCTCCTGC 721 AGAGTGATGG TGATGATTTT AATTACATCA TTGCATTTTT CCTTGGAACA GCAGCCTGCC 781 TTTACCAGTG TTATTTACTT GTCTACTACA CCGGCTGGCG GAATGTCAAA TCTTTTTTGA 841 CTTTTGGCTT AATCTGTCTA TGCAACATGT ATCTCTATGA ACTGCGCAAC CTCTGGCAGC 901 TTTTCTTTCA TGTGACTGTG GGAGCATTTG TTACACTACA GATCTGGCTA AGGCAAGCCC 961 AGGGCAAGGC TCCCGATTAT GATGTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1021 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1081 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGAATTCG 1141 CAGAGGGAAG GGCCGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1201 aagatt