Construct: ORF TRCN0000472852
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009762.1_s317c1
- Derived from:
- ccsbBroadEn_12271
- DNA Barcode:
- TTCGCGTGACGCTTGCGGCCATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TDP1 (55775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472852
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | NM_001330205.2 | 51.4% | 51.2% | (many diffs) |
| 2 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_011536944.2 | 51.4% | 51.2% | (many diffs) |
| 3 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_017021440.2 | 51.4% | 51.2% | (many diffs) |
| 4 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | NM_001008744.2 | 48.9% | 48.6% | (many diffs) |
| 5 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | NM_018319.4 | 48.9% | 48.6% | (many diffs) |
| 6 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_005267848.3 | 48.9% | 48.6% | (many diffs) |
| 7 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_006720197.4 | 48.9% | 48.6% | (many diffs) |
| 8 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_011536942.3 | 48.9% | 48.6% | (many diffs) |
| 9 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_017021439.2 | 48.9% | 48.6% | (many diffs) |
| 10 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449649.1 | 45.7% | 45.5% | (many diffs) |
| 11 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449650.1 | 45.7% | 45.5% | (many diffs) |
| 12 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449651.1 | 45.7% | 45.5% | (many diffs) |
| 13 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449652.1 | 45.7% | 45.5% | (many diffs) |
| 14 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449653.1 | 45.7% | 45.5% | (many diffs) |
| 15 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449654.1 | 45.7% | 45.5% | (many diffs) |
| 16 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449655.1 | 45.7% | 45.5% | (many diffs) |
| 17 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XM_024449656.1 | 45.7% | 45.5% | (many diffs) |
| 18 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XR_001750427.2 | 24.3% | 1_67del;962_3678del | |
| 19 | human | 55775 | TDP1 | tyrosyl-DNA phosphodiestera... | XR_943492.3 | 22.5% | 1_294del;1178_1224del;1236_3956del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcaggaaggc gattatggga ggtggaccat atctagtagt gatgaaagtg 121 aggaagaaaa gccaaaacca gacaagccat ctacctcttc tcttctctgt gccaggcaag 181 gagcagcaaa tgagcccagg tacacctgtt ccgaggccca gaaagctgca cacaagagga 241 aaatatcacc tgtgaaattc agcaatacag attcagtttt acctcccaaa aggcagaaaa 301 gcggttccca ggaggacctc ggctggtgtc tgtccagcag tgatgatgag ctgcaaccag 361 aaatgccgca gaagcaggct gagaaagtgg tgatcaaaaa ggagaaagac atctctgctc 421 ccaatgacgg cactgcccaa agaactgaaa atcatggcgc tcccgcctgc cacaggctca 481 aagaggagga agacgagtat gagacatcag gggagggcca ggacatttgg gacatgctgg 541 ataaagggaa ccccttccag ttttacctca ctagagtctc tGGAGTTAAG CCAAAGTATA 601 ACTCTGGAGC CCTCCACATC AAGGATATTT TATCTCCTTT ATTTGGGACG CTTGTTTCTT 661 CAGCTCAGTT TAACTACTGC TTTGACGTGG ACTGGCTCGT AAAACAGTAT CCACCAGAGT 721 TCAGGAAGAA GCCAATCCTG CTTGTGCATG GTGATAAGCG AGAGGCTAAG GCTCACCTCC 781 ATGCCCAGGC CAAGCCTTAC GAGAACATCT CTCTCTGCCA GGCAAAGTTG GATATTGCGT 841 TTGGAACACA CCACACGAAA ATGATGCTGC TGCTCTATGA AGAAGGCCTC CGGGTTGTCA 901 TACACACCTC CAACCTCATC CATGCTGACT GGCACCAGAA AACTCAAGGA ACTCACCTGT 961 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGATTCGC GTGACGCTTG CGGCCATATA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt