Construct: ORF TRCN0000472855
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014647.1_s317c1
- Derived from:
- ccsbBroadEn_06929
- DNA Barcode:
- AATTACCAAGATATAAATGCTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHD (6392)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472855
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_003002.4 | 99.7% | 100% | 204C>T |
| 2 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_001276506.2 | 78% | 69.5% | (many diffs) |
| 3 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_001276504.2 | 75.2% | 75.4% | 52_53ins117;87C>T |
| 4 | human | 6392 | SDHD | succinate dehydrogenase com... | NM_001276503.2 | 53.4% | 39.6% | 169_170ins145;255_256ins77 |
| 5 | human | 6392 | SDHD | succinate dehydrogenase com... | NR_077060.2 | 34.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 543
- ORF length:
- 477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggttctctgg aggctgagtg ccgtttgcgg tgccctagga ggccgagctc 121 tgttgcttcg aactccagtg gtcagaCCTG CTCATATCTC AGCATTTCTT CAGGACCGAC 181 CTATCCCAGA ATGGTGTGGA GTGCAGCACA TACACTTGTC ACCGAGCCAC CATTCTGGCT 241 CCAAGGCTGC ATCTCTCCAC TGGACTAGTG AGAGGGTTGT CAGTGTTTTG CTCCTGGGTC 301 TGCTTCCGGC TGCTTATTTG AATCCTTGCT CTGCGATGGA CTATTCCCTG GCTGCAGCCC 361 TCACTCTTCA TGGTCACTGG GGCCTTGGAC AAGTTGTTAC TGACTATGTT CATGGGGATG 421 CCTTGCAGAA AGCTGCCAAG GCAGGGCTTT TGGCACTTTC AGCTTTAACC TTTGCTGGGC 481 TTTGCTATTT CAACTATCAC GATGTGGGCA TCTGCAAAGC TGTTGCCATG CTGTGGAAGC 541 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 601 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 661 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TTACCAAGAT ATAAATGCTT GCACGCGTTA 721 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt