Construct: ORF TRCN0000472873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005908.1_s317c1
- Derived from:
- ccsbBroadEn_00420
- DNA Barcode:
- ACCCTGAAACCACCGTTTTAAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAP (1611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1611 | DAP | death associated protein | NM_004394.3 | 100% | 100% | |
| 2 | human | 1611 | DAP | death associated protein | NM_001291963.2 | 40.1% | 27.5% | 151_152ins43;264_612del |
| 3 | mouse | 223453 | Dap | death-associated protein | NM_146057.3 | 86.6% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 396
- ORF length:
- 306
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCTTCGCC TCCCGAAGGG AAACTAGAGA 121 CTAAAGCTGG ACACCCGCCC GCCGTGAAAG CTGGTGGAAT GCGAATTGTG CAGAAACACC 181 CACATACAGG AGACACCAAA GAAGAGAAAG ACAAGGATGA CCAGGAATGG GAAAGCCCCA 241 GTCCACCTAA ACCCACTGTG TTCATCTCTG GGGTCATCGC CCGGGGTGAC AAAGATTTCC 301 CCCCGGCGGC TGCGCAGGTG GCTCACCAGA AGCCGCATGC CTCCATGGAC AAGCATCCTT 361 CCCCAAGAAC CCAGCACATC CAGCAGCCAC GCAAGTGCCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 AACCCTGAAA CCACCGTTTT AAACCACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt