Construct: ORF TRCN0000472878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009491.1_s317c1
- Derived from:
- ccsbBroadEn_14145
- DNA Barcode:
- GATCTGCCTGAATCTACATGATTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ANKMY1 (51281)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001354026.1 | 66.4% | 54.1% | 0_1ins267;613_614insC;876_877ins174 |
2 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_017844.4 | 61% | 40.9% | (many diffs) |
3 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001282780.2 | 60.8% | 40.8% | (many diffs) |
4 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001308375.2 | 51.9% | 34.8% | (many diffs) |
5 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004285.2 | 48.5% | 32.6% | (many diffs) |
6 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NR_148697.2 | 46.5% | (many diffs) | |
7 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_024452955.1 | 43.3% | 27.5% | (many diffs) |
8 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001282771.3 | 42.5% | 28.5% | (many diffs) |
9 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001354023.2 | 42.5% | 28.5% | (many diffs) |
10 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001282781.2 | 42.4% | 35% | (many diffs) |
11 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004289.1 | 39% | 23.1% | (many diffs) |
12 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004288.1 | 38.7% | 23% | (many diffs) |
13 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004275.2 | 38.7% | 26% | (many diffs) |
14 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004276.1 | 38.7% | 26% | (many diffs) |
15 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NR_148696.2 | 38.7% | (many diffs) | |
16 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004287.1 | 38.6% | 22.9% | (many diffs) |
17 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004290.1 | 36.1% | 15% | (many diffs) |
18 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_024452952.1 | 35.7% | 22.3% | (many diffs) |
19 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001354024.2 | 35.5% | 21% | (many diffs) |
20 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_024452954.1 | 34.5% | 21.9% | (many diffs) |
21 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_016552.5 | 33.9% | 20.1% | (many diffs) |
22 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NM_001354025.2 | 33% | 14.1% | (many diffs) |
23 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_024452958.1 | 33% | 14.1% | (many diffs) |
24 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | NR_148698.1 | 31.6% | (many diffs) | |
25 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004281.1 | 30.9% | 18.3% | (many diffs) |
26 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_017004282.1 | 30.9% | 18.3% | (many diffs) |
27 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_024452953.1 | 30.9% | 18.3% | (many diffs) |
28 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_024452956.1 | 27.5% | 11.8% | (many diffs) |
29 | human | 51281 | ANKMY1 | ankyrin repeat and MYND dom... | XM_024452957.1 | 27.5% | 11.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 990
- ORF length:
- 924
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga angggcccan gcctccctta gcttagagga cgaagtctct ggggctggca 121 gccgccaacg cccgctagag gggaagggcg gcgagacccc tgctgccgag gagccggggt 181 ccctgaagaa ctacgctgtc ttcgccacaa gggatgtttc agcagcccct gagaaggaag 241 aggaggaagc tgagggcccg ctgagggcgc aggacctgag ggagtcctac atccagctcg 301 tccagggtgt gcaggagtgg caggatggtt gcatgtacca gggggagttt gggttgaaca 361 tgaagcttgg atatggcaaa ttctcttggc ccacaggcga gtcataccat gggcagtttt 421 accgggacca ctgccatggc ctgggtacct acatgtggcc agatggctcc agtttcacgg 481 gcacatttta cctcagccac cgagaaggct acggcaccat gtacatgaag acacggcttt 541 tccagactca ctgccacaac gacattgtca accttctcct ggactgtggg gccgacgtga 601 acaagtgctc agatgagggt ctcacggcac tcagcatgtg tttcctcctc cactaccccg 661 cccagtcctt caagcccaat gttgctgaac ggaccatacc tgagccccag gaacctccaa 721 aattcccagt tgttccaatc ctttcatcat catttatgga cacaaacctg gagtctctgt 781 actatgaggt gaacgtgcct tcccagggta gctatgagct gaggccaccg ccagcaccac 841 tgctcctgcc acgcgtctca ggcagccacg agggcggcca cttccaggac aCCGGGCAGT 901 GTGGGGGGTC CATAGACCAC AGGAGCAGCT CTCTGAAGGG GGACTCCCCC GTTGGTGAAG 961 GGCAGCCTTG GCCATGTGGA AAGCGGGCTT GAGGACGTGT TGGGAAACAC AGACCGGGGC 1021 AGTCTGTGCA GTGCTGAGAC GAAATTTGAG TCCAACGTGT GTGTGTGCGA CTTCTCCATC 1081 GAGCTCTCGC AGGCCATGCT GGAGAGAAGC GCCCAGTCCC ACAGCTTGCT GAAGATGGCC 1141 TCGCCCTCAC CGTGCACCAG CAGCTTCGAC AAAGGGACCA TGCGGAGGAT GGCGCTGTCC 1201 ATGATCGAGC GGAGGAAGCG CTGGCGGACC ATCAAGCTGC TGCTGCGCCG GGGCGCGGAC 1261 CCCAACCTGT GCTGCGTGCC CATGCAGGTC CTGTTCCTTG CTGTGAAGGC CGGGGACGTG 1321 GATGGGGTGA GGCTGCTGCT GGAGCACGGG GCGAGGACCG ACATCTGCTT TCCGCCGCAG 1381 TGTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 1441 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 1501 TGGCTTTATA TATCTTGTGG AAAGGACGAG ATCTGCCTGA ATCTACATGA TTTACGCGTT 1561 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att