Construct: ORF TRCN0000472879

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015193.1_s317c1
Derived from:
ccsbBroadEn_07856
DNA Barcode:
AAAACCCCTAGATCTGTACGTACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MLC1 (23209)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472879
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23209 MLC1 modulator of VRAC current 1 NM_015166.3 99.9% 99.7% 74C>A
2 human 23209 MLC1 modulator of VRAC current 1 NM_139202.2 99.9% 99.7% 74C>A
3 human 23209 MLC1 modulator of VRAC current 1 XM_017028671.1 99.9% 99.7% 74C>A
4 human 23209 MLC1 modulator of VRAC current 1 XM_011530678.2 85.3% 85.1% 74C>A;894_895ins165
5 human 23209 MLC1 modulator of VRAC current 1 XR_001755181.2 49.1% 1_273del;347C>A;1405_2299del
6 human 23209 MLC1 modulator of VRAC current 1 XR_001755180.2 44.6% 1_505del;579C>A;1637_2531del
7 mouse 170790 Mlc1 megalencephalic leukoenceph... NM_133241.2 82.1% 87.4% (many diffs)
8 mouse 170790 Mlc1 megalencephalic leukoenceph... XM_006520545.3 77.3% 82.3% (many diffs)
9 mouse 170790 Mlc1 megalencephalic leukoenceph... XM_006520544.3 76.2% 81.1% (many diffs)
10 mouse 170790 Mlc1 megalencephalic leukoenceph... XM_011245483.2 76.2% 81.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1197
ORF length:
1131
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac ccaggagcca ttcagagagg agctggccta tgaccggatg cccacgctgg
121agcggggccg gcaagaccac gccagctatg ccccagacgc gaagccgagc gacctgcagc
181tgtcgaagag actgcccccc tgcttcagcc acaagacgtg ggtcttctct gtgctgatgg
241ggagctgcct cctggtgacc tcggggtttt cgctgtacct ggggaacgtg ttcccggctg
301agatggatta cttgcgctgt gctgcaggct cttgcatccc ctcggcaatt gtgagcttca
361ccgtctccag gaggaacgcc aatgtgattc ccaactttca gatattgttt gtttccacgt
421ttgctgtgac cactacgtgt ttaatttggt ttggatgcaa actagtcctg aacccatcag
481caataaacat caacttcaac ctcatcctgc tgctcctgct ggagctgctc atggcggcca
541cggtgatcat cgctgcacgg tccagcgagg aggactgcaa gaaaaagaag ggctccatgt
601ctgacagcgc caacattctg gacgaagtgc catttcctgc tcgggtcctg aaatcttact
661cagtcgtcga ggtaatcgca ggcatctctg ccgtccTCGG GGGGATCATT GCCCTGAACG
721TGGATGACTC AGTTTCAGGC CCACACCTCT CAGTGACGTT CTTTTGGATC CTAGTGGCCT
781GCTTTCCAAG TGCCATTGCC AGTCATGTGG CAGCAGAGTG TCCCAGCAAG TGTCTGGTGG
841AGGTCCTGAT TGCCATAAGC AGCCTCACGT CTCCGCTGCT GTTCACAGCC TCTGGATATC
901TGTCATTCAG CATCATGAGA ATCGTGGAGA TGTTTAAGGA TTACCCGCCA GCCATAAAAC
961CATCCTACGA TGTGCTGCTG CTGCTGCTGC TGCTAGTGCT CCTGCTGCAG GCCGGCCTCA
1021ACACGGGCAC CGCCATCCAG TGCGTGCGCT TCAAGGTCAG TGCAAGGCTG CAGGGTGCAT
1081CCTGGGACAC CCAGAACGGC CCGCAGGAGC GCCTGGCTGG GGAGGTGGCC AGGAGCCCCC
1141TGAAGGAGTT CGACAAGGAG AAAGCCTGGA GAGCCGTCGT GGTGCAAATG GCCCAGTGCC
1201CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1261TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1321ATATATCTTG TGGAAAGGAC GAAAAACCCC TAGATCTGTA CGTACCACGC GTTAAGTCga
1381caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)