Construct: ORF TRCN0000472886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002804.1_s317c1
- Derived from:
- ccsbBroadEn_02868
- DNA Barcode:
- CATCCAATGCCTTGGTCATACCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- METTL7A (25840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472886
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25840 | METTL7A | methyltransferase like 7A | NM_014033.4 | 100% | 100% | |
| 2 | mouse | 70152 | Mettl7a1 | methyltransferase like 7A1 | XM_006521389.1 | 78.5% | 80.7% | (many diffs) |
| 3 | mouse | 70152 | Mettl7a1 | methyltransferase like 7A1 | XM_006521390.3 | 70% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcttaccatc tttatcctga gactggccat ttacatcctg acatttccct 121 tgtacctgct gaactttctg ggcttgtgga gctggatatg caaaaaatgg ttcccctact 181 tcttggtgag gttcactgtg atatacaacg aacagatggc aagcaagaag cgggagctct 241 tcagtaacct gcaggagttt gcgggcccct ccgggaaact ctcccTGCTG GAAGTGGGCT 301 GTGGCACGGG GGCCAACTTC AAGTTCTACC CACCTGGGTG CAGGGTGACC TGTATTGACC 361 CCAACCCCAA CTTTGAGAAG TTTTTGATCA AGAGCATTGC AGAGAACCGA CACCTGCAGT 421 TTGAGCGCTT TGTGGTAGCT GCCGGGGAGA ACATGCACCA GGTGGCTGAT GGCTCTGTGG 481 ATGTGGTGGT CTGCACCCTG GTGCTGTGCT CTGTGAAGAA CCAGGAGCGG ATTCTCCGCG 541 AGGTGTGCAG AGTGCTGAGA CCGGGAGGGG CTTTCTATTT CATGGAGCAT GTGGCAGCTG 601 AGTGTTCGAC TTGGAATTAC TTCTGGCAAC AAGTCCTGGA TCCTGCCTGG CACCTTCTGT 661 TTGATGGGTG CAACCTGACC AGAGAGAGCT GGAAGGCCCT GGAGCGGGCC AGCTTCTCTA 721 AGCTGAAGCT GCAGCACATC CAGGCCCCAC TGTCCTGGGA GTTGGTGCGC CCTCATATCT 781 ATGGATATGC TGTGAAATAC CCCACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACATCCAA TGCCTTGGTC 961 ATACCGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt