Transcript: Mouse XM_006521389.1

PREDICTED: Mus musculus methyltransferase like 7A1 (Mettl7a1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mettl7a1 (70152)
Length:
1898
CDS:
97..777

Additional Resources:

NCBI RefSeq record:
XM_006521389.1
NBCI Gene record:
Mettl7a1 (70152)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006521389.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000097560 CGTGATGTACAATGAGCAGAT pLKO.1 174 CDS 100% 4.050 2.835 N Mettl7a1 n/a
2 TRCN0000335663 CGTGATGTACAATGAGCAGAT pLKO_005 174 CDS 100% 4.050 2.835 N Mettl7a1 n/a
3 TRCN0000097561 GAAGCTAAAGCTACAGCACAT pLKO.1 696 CDS 100% 4.050 2.835 N Mettl7a1 n/a
4 TRCN0000335665 GAAGCTAAAGCTACAGCACAT pLKO_005 696 CDS 100% 4.050 2.835 N Mettl7a1 n/a
5 TRCN0000097563 CGGAGCCAACTTCAAGTTCTA pLKO.1 285 CDS 100% 4.950 2.970 N Mettl7a1 n/a
6 TRCN0000335664 CGGAGCCAACTTCAAGTTCTA pLKO_005 285 CDS 100% 4.950 2.970 N Mettl7a1 n/a
7 TRCN0000347708 GAACGGTCTACCTGGAATTAC pLKO_005 577 CDS 100% 13.200 6.600 Y Mettl7a3 n/a
8 TRCN0000097569 CCCGGTAAGAAGGAATCAGAA pLKO.1 1100 3UTR 100% 4.950 2.475 Y Mettl7a2 n/a
9 TRCN0000097559 CCGAATAAATAAATCCCAGAA pLKO.1 1027 3UTR 100% 4.050 2.025 Y Mettl7a1 n/a
10 TRCN0000183893 CTTCAGCAATCTGCAGGAGTT pLKO.1 216 CDS 100% 4.050 2.025 Y Methig1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006521389.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02868 pDONR223 100% 78.5% 80.7% None (many diffs) n/a
2 ccsbBroad304_02868 pLX_304 0% 78.5% 80.7% V5 (many diffs) n/a
3 TRCN0000472886 CATCCAATGCCTTGGTCATACCGG pLX_317 69.5% 78.5% 80.7% V5 (many diffs) n/a
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