Construct: ORF TRCN0000472888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017051.1_s317c1
- Derived from:
- ccsbBroadEn_09967
- DNA Barcode:
- TGCCCTGTTCAATGTCAATACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RUSC1-AS1 (284618)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284618 | RUSC1-AS1 | RUSC1 antisense RNA 1 | NR_145426.1 | 42.3% | 1_306del;933T>G;1015_1670del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 777
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccggga gggtcagaga acgcggctgc gctctggatc tccgaagggg 121 ggcgggggcc tggaagaggt ccgggcccag aatggacctc caggtccctg ctgccgcaat 181 ctggccctgc cctccagcct accccttact cccagaggaa gggacccagg gagacccacc 241 cagatgccct gaaaggaggt ggaggatggg ggtggggcaa tacgcagagt ttgtctggtg 301 aatgcaggaa aggggtgggg gctggagagg aaaaggatgg agcagccgtc agcttgtcaa 361 ctccgcatct gctggctgcc tcggccgggc tccagccTGC GCCCTCGCCC CTCGGAACAG 421 CGGTATGTCC ATTCTCTCCG CATTCGTCTC CATCGTTCAG CCACCACCGC ACCCTTTCTC 481 TATTCATTTC TCCTGCTCCC TTGTCCTGCC CCGCCCCTCG TGCCCAGGTC CATCGGTCTA 541 CACCCATGGG CCGAGCCCTC CTCACCAGGG TCCTCCTGGA ACCGCTCCGG CCTTGGGCTT 601 GTCCGCGGCT CCCACGGTCC CCGCCCGGCG GGGCACAGAG CGGGAGGGGA GGGGCACTAG 661 CACAACCTAC CCTGCGCTGC GCAGCTGCAC CCCTGCGCGC ATGGGCGTGG CGTAGCTCAG 721 ACCCGCCCCC AGCGTTTAGC GTCTTTTGTC ACCCACCTAG AGGGTTTGAT ATATCCTTGC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GATGCCCTGT TCAATGTCAA TACCTTACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt