Construct: ORF TRCN0000472889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006590.1_s317c1
- Derived from:
- ccsbBroadEn_14233
- DNA Barcode:
- AAAAGGGGCCAAGTGGTGGGCACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CEP126 (57562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57562 | CEP126 | centrosomal protein 126 | NM_020802.4 | 99.8% | 65.4% | (many diffs) |
| 2 | human | 57562 | CEP126 | centrosomal protein 126 | NM_001363543.2 | 82% | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2271
- ORF length:
- 2202
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggcgggg aggcccggaa cccggagcgc ggtcggggaa ctgggcactg 121 aatcatcgga caacctcgac agagcccccc tcggccctcg ggagagcggc gggcatcacc 181 gacctggctc ttacctagat atgaaaatcc atctggagaa aaatttagaa gaagagcgcc 241 agatattact gcagcaacaa aaaatatgtc gaaatcgagc acgtaaatat tttgtggagt 301 caaatcggag aaaaaaagct tttgaggaga aacgaaaaga acaggaagaa aaagaacacc 361 aaattagaga acaaatactt caacaaagaa aacagaagtt tgaagaagtt actgaaaaat 421 tccagcgtgc ccatgttcct ctttcacagc ggaggaaagc agtttcccga aaaccagttc 481 ctccattaga agaggccctc aaacaaattc aggaatccaa cttaaaatca gaagtaaacc 541 ttcccttttc ccgtagacca acaataaact ggagagctat agattctgcc ttgccttccg 601 cattatcaaa aaatgatcac aagcatcaga aacaactctt atccaaaatc aattgtgaga 661 aagaaatgaa tgaaaacatg agggcaacct tggctactag caaaaatgtg ttccagctta 721 aactggagga aactcagaaa ctcctcgaag atcaacatct aagcaatttg caaaaatttt 781 gtgatgaagt taatcagata accaattctg aaaccctctc aagtatagac agtcttgagg 841 ctacagagca tgaagaaata tatttaacac ttaataagga gcattccaca tccatccagc 901 ggaataccat ttccctcaaa ccagcaaata tgcaatcaac taatctcagc tgctttgatg 961 aagataaact ggcattctct aaaactcaac atataaataa ttggcttaca aatttagatg 1021 cttcaaatac tcagaatgtc acagctttct cagatatttt aagtaaatct aatgttctgc 1081 cttcatggga atattttaat agtaaagaac aaaatccatc tcctttgaat ggaacagtgg 1141 aaagagccac aaatactgct aataattcag taccctttgt atctagccca cccatgtttg 1201 tactagataa aaaatgtgaa aagacctctg aaactagcac tatgaggaca actgactcca 1261 cttctggagc attcaaaaga gagagaccat tagttactga gagcccaaca tttaaattta 1321 gcaaatccca aagcacttca gattctctaa cccaggaagt ggctacattt ccagaccaag 1381 agaaatattc tgaattaaat caagaaaatg gaactacttc aattcctact tcatgtgtac 1441 cagtggcaac gcctttagtt ttgccatcta atatacagtc agctagacct tcagcaaaga 1501 acagtataca cataaaagaa attgatgcag tgcagtgttc tgataagtta gatgaattga 1561 aagatggtaa agaagaagag ataaaatatt ttaattgcaa taaggaagag ttgcctttat 1621 tttcagacag ttttcaagat gcctatatac ctcacaatcc tgattcaaaa gatgaaaaac 1681 aaaaattagc tgaaacatca tccttgtcta atgtaacttc taattatgac tttgttggcc 1741 agcataagaa aatgaaatac aacatccatg agagaaatgg tgtgagattt cttaaaagta 1801 ttttaaagaa agaatctaaa tatgaacatg gttatcttaa ggcattaatt ataaatcaga 1861 gctttaagtt tggaaatcaa aaagcagcag ctatcagaga tagtattgaa ttaacaaagg 1921 aaaaaggtgc agaaattcca aagactatta aaaaactgag gtggtttgat gaaactagca 1981 atatagaaaa caatgctgaa aacagtcatt cactgaagaa taaaacagga acaactcaac 2041 agcattctca acaattccac attcaaagtg gtgctggaag caacataatt agtgtttcta 2101 cttgtgctgt aaattctgct gatacaaaga agtccaggga ggattctatc tctgaaaatg 2161 ttacgacttt aggaggatct ggagcagacc atatgccttt gaactgtttt ataccttcag 2221 gttataactt tgctaaacat gcctggccag cctcaaaaaa aagaagaaag taaaatccct 2281 gtacatgatg attctaaaac taagcaaggt aagccacaaa gaggtagagc aaaaataatt 2341 agaaaaccag gatctgcaaa agtccaatca ggctttatat gtacaaacag aaaaggcgct 2401 gtcattcaac cacagtctgc aagcaaagtc aacatattta cacaagctca gggaaaatta 2461 attatacctt gtcctcctcc tcaatctaca tcaaatatta gaagtggtaa aaatatacaa 2521 gtgtctcagt gtcaaccagt aactcctgaa aatcctcaaa acattattac acataactct 2581 tttaattcaa aacatgtgct tccaacagaa cacagtttga atcagtggaa tcaggaaagt 2641 agttctccac tctcaaatgc ttgttctgac ctagtcactg tgataccatc actgccatca 2701 tattgttctt cagagtgcca aactttcgca aaaataaatc attcaaatgg cactcaagca 2761 gttgcccggc aagatgcgac attatattgc acccaaagaa gtcctgtttg tgaagaaagt 2821 tatccgtctg tgactctaag aactgctgaa gaagaatcag ttcccttatg gaaaagaggt 2881 cctaatgtcc tgcatcaaaa taagagggct acagggtcta ctgttatgag aagaaaacga 2941 attgctgaaa ctaagcggag aaatatttta gagcagaaaa gacaaaaccc tggatctgta 3001 ggacagaagt acagtgagca aattaataat tttggacaaa gtatcctgct aagttcaagt 3061 gagccaaaaC AAACTACAAG GGGTACTTCT TATATTGAAG AAGTTTCAGA TAGTACTTCT 3121 GAGTTTTTGA TGGCTGAAAA CTTAGTGAAA GCATCAGTGC CGGAGGATGA GATTCTGACT 3181 GTCTTGAATA GCAAACAGAT ACAGAAATCA AATCTACCTT TAAATAAAAC TCAACAATTC 3241 AACATCTGCA CACTGTCAGC TGAAGAACAG AAGATCCTAG AGTCCCTTAA TGATCTCAAT 3301 GAAAGACTAC ATTATATACA AGAATCCATT TGCAAAAACC CATCCATCAA AAATACTTTA 3361 CAAATAATAC CACTTCTGGA GAAGAGAGAA GATAGAACCA GCAGCTGCAG AGACAAGAGA 3421 TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 3481 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 3541 CTTTATATAT CTTGTGGAAA GGACGAAAAA GGGGCCAAGT GGTGGGCACG ACGCGTTAAG 3601 TCgacaatca acctctggat tacaaaattt gtgaaagatt