Construct: ORF TRCN0000472906
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003097.1_s317c1
- Derived from:
- ccsbBroadEn_04316
- DNA Barcode:
- ACTGTACGCAAGCCAGGCGCTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B3GNT5 (84002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472906
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | NM_032047.5 | 100% | 100% | |
| 2 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_005247823.5 | 100% | 100% | |
| 3 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_005247824.3 | 100% | 100% | |
| 4 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_005247825.3 | 100% | 100% | |
| 5 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_011513225.2 | 100% | 100% | |
| 6 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_011513226.2 | 100% | 100% | |
| 7 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_011513227.2 | 100% | 100% | |
| 8 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_011513228.2 | 100% | 100% | |
| 9 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_011513229.2 | 100% | 100% | |
| 10 | human | 84002 | B3GNT5 | UDP-GlcNAc:betaGal beta-1,3... | XM_017007309.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1200
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aatgttggtt agtggcagaa gagtcaaaaa atggcagtta attattcagt 121 tatttgctac ttgtttttta gcgagcctca tgtttttttg ggaaccaatc gataatcaca 181 ttgtgagcca tatgaagtca tattcttaca gatacctcat aaatagctat gactttgtga 241 atgataccct gtctcttaag cacacctcag cggggcctcg ctaccaatac ttgattaacc 301 acaaggaaaa gtgtcaagct caagacgtcc tccttttact gtttgtaaaa actgctcctg 361 aaaactatga tcgacgttcc ggaattagaa ggacgtgggg caatgaaaat tatgttcggt 421 ctcagctgaa tgccaacatc aaaactctgt ttgccttagg aactcctaat ccactggagg 481 gagaagaact acaaagaaaa ctggcttggg aagatcaaag gtacaatgat ataattcagc 541 aagactttgt tgattctttc tacaatctta ctctgaaatt acttatgcag ttcagttggg 601 caaataccta ttgtccacat gccaaatttc ttatgactgc tgatgatgac atatttattc 661 acatgccaaa tctgattgag taccttcaaa gtttagaaca aattggtgtt caagactttt 721 ggattggtcg tgttcatcgt ggtgccccTC CCATTAGAGA TAAAAGCAGC AAATACTACG 781 TGTCCTATGA AATGTACCAG TGGCCAGCTT ACCCTGACTA CACAGCCGGA GCTGCCTATG 841 TAATCTCCGG TGATGTAGCT GCCAAAGTCT ATGAGGCATC ACAGACACTA AATTCAAGTC 901 TTTACATAGA CGATGTGTTC ATGGGCCTCT GTGCCAATAA AATAGGGATA GTACCGCAGG 961 ACCATGTGTT TTTTTCTGGA GAGGGTAAAA CTCCTTATCA TCCCTGCATC TATGAAAAAA 1021 TGATGACATC TCATGGACAC TTAGAAGATC TCCAGGACCT TTGGAAGAAT GCTACAGATC 1081 CTAAAGTAAA AACCATTTCC AAAGGTTTTT TTGGTCAAAT ATACTGCAGA TTAATGAAGA 1141 TAATTCTCCT TTGTAAAATT AGCTATGTGG ACACATACCC TTGTAGGGCT GCGTTTATCT 1201 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1321 TTTATATATC TTGTGGAAAG GACGAACTGT ACGCAAGCCA GGCGCTGTGA CGCGTTAAGT 1381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt