Construct: ORF TRCN0000472913

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017170.1_s317c1
Derived from:
ccsbBroadEn_13966
DNA Barcode:
TCATACTTCACTGCCTTGGATCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TEAD3 (7005)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472913
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7005 TEAD3 TEA domain transcription fa... NM_003214.4 74.4% 74.2% 1_333del;1291A>G
2 mouse 21678 Tead3 TEA domain family member 3 NM_001204156.1 66.4% 71.9% (many diffs)
3 mouse 21678 Tead3 TEA domain family member 3 NM_011566.4 66.4% 71.9% (many diffs)
4 mouse 21678 Tead3 TEA domain family member 3 NM_001098226.3 62.7% 67.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1038
ORF length:
972
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa cctggaccag gtctccaagg acaaagccct tcagagcatg gcgtccatgt
121cctctgccca gatcgtctct gccagtgtcc tgcagaacaa gttcagccca ccttcccctc
181tgccccaggc cgtcttctcc acttcctcgc ggttctggag cagcccccct ctcctgggac
241agcagcctgg accctctcag gacatcaagc cctttgcaca gccagcctac cccatccagc
301cgcccctgcc gccgacgctc agcagttatg agcccctggc cccgctcccc tcagctgctg
361cctctgtgcc tgtgtggcag gaccgtacca ttgcctcctc ccggctgcgg ctcctggagt
421attcagcctt catggaggtg cagcgagacc ctgacacgta cagcaaacac ctgtttgtgc
481acatcggcca gacgaacccc gccttctcag acccacccct ggaggcagta gatgtgcgcc
541agatctatga caaattcccc gagaaaaagg gaggattgaa ggagctctat gagaaggggc
601cccctaatgC CTTCTTCCTT GTCAAGTTCT GGGCCGACCT CAACAGCACC ATCCAGGAGG
661GCCCGGGAGC CTTCTATGGG GTCAGCTCTC AGTACAGCTC TGCTGATAGC ATGACCATCA
721GCGTCTCCAC CAAGGTGTGC TCCTTTGGCA AACAGGTGGT AGAGAAGGTG GAGACTGAGT
781ATGCCAGGCT GGAGAACGGG CGCTTTGTGT ACCGTATCCA CCGCTCGCCC ATGTGCGAGT
841ACATGATCAA CTTCATCCAC AAGCTGAAGC ACCTGCCCGA GAAGTACATG ATGAACAGCG
901TGCTGGAGAA CTTCACCATC CTGCAGGTGG TCACGAGCCG GGACTCCCAG GAGACCCTGC
961TTGTCATTGC TTTTGTCTTC GAAGTCTCCA CCAGTGAGCA CGGGGCCCAG CACCATGTCT
1021ACGAGCTCGT CAAAGACTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1081TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1141GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCATACT TCACTGCCTT
1201GGATCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)