Construct: ORF TRCN0000472913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017170.1_s317c1
- Derived from:
- ccsbBroadEn_13966
- DNA Barcode:
- TCATACTTCACTGCCTTGGATCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEAD3 (7005)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472913
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7005 | TEAD3 | TEA domain transcription fa... | NM_003214.4 | 74.4% | 74.2% | 1_333del;1291A>G |
| 2 | mouse | 21678 | Tead3 | TEA domain family member 3 | NM_001204156.1 | 66.4% | 71.9% | (many diffs) |
| 3 | mouse | 21678 | Tead3 | TEA domain family member 3 | NM_011566.4 | 66.4% | 71.9% | (many diffs) |
| 4 | mouse | 21678 | Tead3 | TEA domain family member 3 | NM_001098226.3 | 62.7% | 67.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cctggaccag gtctccaagg acaaagccct tcagagcatg gcgtccatgt 121 cctctgccca gatcgtctct gccagtgtcc tgcagaacaa gttcagccca ccttcccctc 181 tgccccaggc cgtcttctcc acttcctcgc ggttctggag cagcccccct ctcctgggac 241 agcagcctgg accctctcag gacatcaagc cctttgcaca gccagcctac cccatccagc 301 cgcccctgcc gccgacgctc agcagttatg agcccctggc cccgctcccc tcagctgctg 361 cctctgtgcc tgtgtggcag gaccgtacca ttgcctcctc ccggctgcgg ctcctggagt 421 attcagcctt catggaggtg cagcgagacc ctgacacgta cagcaaacac ctgtttgtgc 481 acatcggcca gacgaacccc gccttctcag acccacccct ggaggcagta gatgtgcgcc 541 agatctatga caaattcccc gagaaaaagg gaggattgaa ggagctctat gagaaggggc 601 cccctaatgC CTTCTTCCTT GTCAAGTTCT GGGCCGACCT CAACAGCACC ATCCAGGAGG 661 GCCCGGGAGC CTTCTATGGG GTCAGCTCTC AGTACAGCTC TGCTGATAGC ATGACCATCA 721 GCGTCTCCAC CAAGGTGTGC TCCTTTGGCA AACAGGTGGT AGAGAAGGTG GAGACTGAGT 781 ATGCCAGGCT GGAGAACGGG CGCTTTGTGT ACCGTATCCA CCGCTCGCCC ATGTGCGAGT 841 ACATGATCAA CTTCATCCAC AAGCTGAAGC ACCTGCCCGA GAAGTACATG ATGAACAGCG 901 TGCTGGAGAA CTTCACCATC CTGCAGGTGG TCACGAGCCG GGACTCCCAG GAGACCCTGC 961 TTGTCATTGC TTTTGTCTTC GAAGTCTCCA CCAGTGAGCA CGGGGCCCAG CACCATGTCT 1021 ACGAGCTCGT CAAAGACTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCATACT TCACTGCCTT 1201 GGATCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt