Construct: ORF TRCN0000472924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014793.1_s317c1
- Derived from:
- ccsbBroadEn_08670
- DNA Barcode:
- CCTTTTCGAACATGATCGATGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP22 (56940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_017011062.1 | 65% | 65.3% | 1_129del;336G>A |
| 2 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_011514757.2 | 51% | 47.9% | (many diffs) |
| 3 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_020185.4 | 43.8% | 44% | 1_309del;516G>A |
| 4 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_001286555.1 | 36.2% | 33.9% | (many diffs) |
| 5 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104475.1 | 6.7% | (many diffs) | |
| 6 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104474.1 | 6.5% | (many diffs) | |
| 7 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104473.1 | 6.5% | (many diffs) | |
| 8 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | NM_134068.3 | 38.9% | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 274
- ORF end:
- 517
- ORF length:
- 243
- Sequence:
-
1 GGCACCTCGA TTAGTTCTCG AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT 61 TTTATGCGAT GGAGTTTCCC CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC 121 ACTTGATGTA ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA 181 AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC 241 GATTGATCAA CAAGTTTGTA CAAAAAAGTT GGCATGACCG TCACTGACTT TGGCTGGGAG 301 GATGCCCTGC ACACCGTGCG TGCTGGGAGA TCCTGTGCCA ACCCCAACGT GGGCTTCCAG 361 AGACAGCTCC AGGAGTTTGA GAAGCATGAG GTCCATCAGT ATCGGCAGTG GCTGAAGGAA 421 GAATATGGAG AGAGCCCTTT GCAGGATGCA GAAGAAGCCA AAAACATTCT GGCCGCTCCA 481 GGAATTCTGA AGTTCTGGGC CTTTCTCAGA AGACTGTACC CAACTTTCTT GTACAAAGTG 541 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 601 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 661 GACCTTTTCG AACATGATCG ATGCCAACGC GTTAAGTCga caatcaacct ctggattaca 721 aaatttgtga aagatt