Construct: ORF TRCN0000472928
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001037.3_s317c1
- Derived from:
- ccsbBroadEn_15287
- DNA Barcode:
- TGTTTACCAACACTAACACTGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HIPK1 (204851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472928
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 204851 | HIPK1 | homeodomain interacting pro... | NM_181358.2 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 204851 | HIPK1 | homeodomain interacting pro... | NM_198269.2 | 97.3% | 96.8% | (many diffs) |
| 3 | human | 204851 | HIPK1 | homeodomain interacting pro... | XM_011540977.3 | 68.8% | 66.6% | (many diffs) |
| 4 | human | 204851 | HIPK1 | homeodomain interacting pro... | NM_001369806.1 | 67.2% | 66.9% | (many diffs) |
| 5 | human | 204851 | HIPK1 | homeodomain interacting pro... | NM_198268.3 | 67.2% | 66.9% | (many diffs) |
| 6 | human | 204851 | HIPK1 | homeodomain interacting pro... | XM_005270613.4 | 67.2% | 66.9% | (many diffs) |
| 7 | human | 204851 | HIPK1 | homeodomain interacting pro... | XM_024453908.1 | 67.2% | 66.9% | (many diffs) |
| 8 | human | 204851 | HIPK1 | homeodomain interacting pro... | NM_001369807.1 | 67.1% | 66.8% | (many diffs) |
| 9 | human | 204851 | HIPK1 | homeodomain interacting pro... | XM_005270610.5 | 67.1% | 66.8% | (many diffs) |
| 10 | human | 204851 | HIPK1 | homeodomain interacting pro... | XM_005270611.5 | 65.2% | 64.8% | (many diffs) |
| 11 | human | 204851 | HIPK1 | homeodomain interacting pro... | XM_006710443.4 | 64.4% | 64.1% | (many diffs) |
| 12 | human | 204851 | HIPK1 | homeodomain interacting pro... | NM_152696.4 | 54.9% | 52.9% | (many diffs) |
| 13 | mouse | 15257 | Hipk1 | homeodomain interacting pro... | NM_001301304.1 | 61.3% | 65% | (many diffs) |
| 14 | mouse | 15257 | Hipk1 | homeodomain interacting pro... | NM_010432.2 | 61.3% | 65% | (many diffs) |
| 15 | mouse | 15257 | Hipk1 | homeodomain interacting pro... | XM_011240024.1 | 58.6% | 62.2% | (many diffs) |
| 16 | mouse | 15257 | Hipk1 | homeodomain interacting pro... | NM_001301306.1 | 57.7% | 61.3% | (many diffs) |
| 17 | mouse | 15257 | Hipk1 | homeodomain interacting pro... | NR_125731.1 | 47.7% | (many diffs) | |
| 18 | mouse | 15257 | Hipk1 | homeodomain interacting pro... | XR_375491.3 | 28.1% | (many diffs) | |
| 19 | mouse | 15257 | Hipk1 | homeodomain interacting pro... | XR_001783665.1 | 26.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2514
- ORF length:
- 2448
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tttgatgttt cagattcgtt atatttcaca aacacaaggc ttgccagctg 121 aatatcttct cagtgccgga acaaaaacaa ccaggttttt caacagagat cctaatttgg 181 ggtacccact gtggaggctt aagacacctg aagaacatga actggagact ggaataaaat 241 caaaagaagc tcggaagtac atttttaatt gcttagatga catggctcag gtgaatatgt 301 ctacggacct ggagggaaca gacatgttgg cagagaaggc agaccgaaga gaatacattg 361 atctgttaaa gaaaatgctc acaattgatg cagataagag aattacccct ctaaaaactc 421 ttaaccatca gtttgtgaca atgactcacc ttttggattt tccacatagc aatcatgtta 481 agtcttgttt tcagaacatg gagatctgca agcggagggt tcacatgtat gatacagtga 541 gtcagatcaa gagtcccttc actacacatg ttgccccaaa tacaagcaca aatctaacca 601 tgagcttcag caatcagctc aatacagtgc acaatcaggc cagtgttcta gcttccagtt 661 ctactgcagc agctgctact ctttctctgg ctaattcaga tgtctcacta ctaaactacc 721 agtcagcttt gtacccatca tctgctgcac cagttcctgg agttgcccag cagggtgttt 781 ccttgcagcc tggaaccacc cagatttgca ctcagacaga tccattccaa cagacattta 841 tagtatgtcc acctgcgttt caaactggac tacaagcaac aacaaagcat tctggattcc 901 ctgtgaggat ggataatgct gtaccaattg taccccaggc accagctgct cagccactac 961 agattcagtc aggagttctc acgcagggaa gctgtacacc actaatggta gcaactctcc 1021 accctcaagt agccaccatc acaccgcagt atgcggtgcc ctttactctg agctgcgcag 1081 ccggccggcc ggcgctggtt gaacagactg ccgctgtact gcaggcgtgg cctggaggga 1141 ctcagcaaat tctcctgcct tcaacttggc aacagttgcc tggggtagct ctacacaact 1201 ctgtccagcc cacagcaatg attccagagg ccatggggag tggacagcag ctagctgact 1261 ggaggaatgc ccactctcat ggcaaccagt acagcactat catgcagcag ccatccttgc 1321 tgactaacca tgtgacattg gccactgctc agcctctgaa tgttggtgtt gcccatgttg 1381 tcagacaaca acaatccagt tccctccctt cgaagaagaa taagcagtca gctccagtct 1441 cttccaagtc ctctctagat gttctgcctt cccaagtcta ttctctggtt gggagcagtc 1501 ccctccgcac cacatcttct tataattcct tggtccctgt ccaagatcag catcagccca 1561 tcatcattcc agatactccc agccctcctg tgagtgtcat cactatccga agtgacactg 1621 atgaggaaga ggacaacaaa tacaagccca gtagctctgg actgaagcca aggtctaatg 1681 tcatcagtta tgtcactgtc aatgattctc ctgactctga ctcttctttg agcagccctt 1741 attccactga taccctgagt gctctccgag gcaatagtgg atccgttttg gaggggcctg 1801 gcagagttgt ggcagatggc actggcaccc gcactatcat tgtgcctcca ctgaaaactc 1861 agcttggtga ctgcactgta gcaacccagg cctcaggtct cctgagcaat aagactaagc 1921 cagtcgcttc agtgagtggg cagtcatctg gatgctgtat cacccccaca gggtatcgag 1981 ctcaacgcgg ggggaccagt gcagcacaac cactcaatct tagccagaac cagcagtcat 2041 cggtggctcc aacctcacag gagagaagca gcaacccagc cccccgcagg cagcaggcgt 2101 ttgtggcccc tctctcccaa gccccctaca CCTTCCAGCA TGGCAGCCCG CTACACTCGA 2161 CAGGGCACCC ACACCTTGCC CCGGCCCCTG CTCACCTGCC AAGCCAGGCT CATCTGTATA 2221 CGTATGCTGC CCCGACTTCT GCTGCTGCAC TGGGCTCAAC CAGCTCCATT GCTCATCTTT 2281 TCTCCCCACA GGGTTCCTCA AGGCATGCTG CAGCCTATAC CACTCACCCT AGCACTTTGG 2341 TGCACCAGGT CCCTGTCAGT GTTGGGCCCA GCCTCCTCAC TTCTGCCAGC GTGGCCCCTG 2401 CTCAGTACCA ACACCAGTTT GCCACCCAAT CCTACATTGG GTCTTCCCGA GGCTCAACAA 2461 TTTACACTGG ATACCCGCTG AGTCCTACCA AGATCAGCCA GTATTCCTAC TTATACCCAA 2521 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2581 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2641 TATCTTGTGG AAAGGACGAT GTTTACCAAC ACTAACACTG TCGACGCGTT AAGTCgacaa 2701 tcaacctctg gattacaaaa tttgtgaaag att