Construct: ORF TRCN0000472932
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018851.3_s317c1
- Derived from:
- ccsbBroadEn_15137
- DNA Barcode:
- CCAACATCGTACAAGCGGTACTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- IPPK (64768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472932
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64768 | IPPK | inositol-pentakisphosphate ... | NM_022755.6 | 69% | 6.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 624
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatctcaaa ggaaagatgc caaataagat gattcgaaaa ggtgtgttca 121 aagatcagca ttttgatcaa aatctcaact tcatgtacat agaagttgat aaagtaacag 181 agagggagaa agttactgtt atgagcacca ttaatccaac taaggacctg ttggctgact 241 tgattgggtg ccagagactt cctgaagacc aacgtaagaa agtacaccag ctaaaggact 301 tgttggacca gattctgatg ttggacccag ctaaacgaat tagcatcaac caggccctac 361 agcatgcctt catccaggaa aaaattttgc caactttctt gtacaaagtt ggcattataa 421 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca 481 ttatttgcca tccagctgat atcnnctata gtgantcnnn ttacatggnc atagctgttt 541 cctggcagct ctggcccgtg tctcaaaatc ttagaatgtt acacaacatg tcccatggaa 601 caaagaattt cccctcaagt ccataaaaaa atttgaaatc tcatgcgtag cgagacaaca 661 ggagagcccc gatatccctg caatcattat tacaagttat ccgaattgca cagacgcttt 721 catcccttcc atctcgatag gtgcaaataa aagcaaagta tgcaatcatt atgagaagaa 781 atgacacgtc gattacatcc tgccttactg gcttccagca aaagttagcc attacttaac 841 ttatgtgtcg tgagcatagt tctcgctgca aagatgcccg gagccccgtg gctgactgga 901 gcgagcttgc acaccacctg aagccgttct tcttcccttc caacggcctg gccagtgggc 961 cccactgcac aagggctgtg atcagggagc tggtgcacgt gatcacacgg gtgctgctga 1021 gtggctcgga caagggccgg gcaggcaccc tgagtccggg gctcgggcct cagggcccgc 1081 gagtctgcga agccagccct ttcagtagga gccttcgctg ccaaggaaaa aacaccccag 1141 agcgctcggg gttaccgaag ggctgtcttc tgtacaaaac cctccaggtg cagatgttgg 1201 acctgctgga catcgaaggc ctctaccctc tgtacaaccg ggttgagcga tacctggaag 1261 agtttcccga ggagagaaaa accttacaaa tagatgggCC TTATGATGAA GCATTTTACC 1321 AGAAGCTGCT TGACCTTTCC ACTGAGGATG ACGGGACAGT GGCCTTCGCG CTAACGAAGG 1381 TGCAGCAGTA CCGCGTCGCC ATGACTGCCA AGGACTGCTC CATCATGATT GCACTGTCTC 1441 CCTGTCTGCA GGATGCCAGC TCTGATCAAA GGCCTGTCGT CCCTTCATCG AGGTCCAGGT 1501 TTGCCTTTTC CGTGTCTGTG CTGGACCTTG ACCTCAAGCC CTACGAGAGC ATTCCCCATC 1561 AGTATAAACT GGACGGCAAG ATCGTCAACT ATTATTCAAA GACTGTACGT GCCAAAGACA 1621 ACGCCGTGAT GTCGACTCGG TTCAAGGAAA GCGAAGATTG CACATTAGTT CTCCACAAGG 1681 TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1741 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1801 GGCTTTATAT ATCTTGTGGA AAGGACGACC AACATCGTAC AAGCGGTACT TGACGCGTTA 1861 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt