Construct: ORF TRCN0000472935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000814.1_s317c1
- Derived from:
- ccsbBroadEn_09963
- DNA Barcode:
- GCTTTTTGACGATCAATCTAGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFP82 (284406)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284406 | ZFP82 | ZFP82 zinc finger protein | NM_133466.4 | 99.9% | 100% | 1134T>C |
| 2 | human | 284406 | ZFP82 | ZFP82 zinc finger protein | NM_001321917.2 | 99.7% | 99.8% | 136_138delGCA;1137T>C |
| 3 | human | 284406 | ZFP82 | ZFP82 zinc finger protein | NM_001321919.2 | 18.3% | 15.7% | (many diffs) |
| 4 | human | 284406 | ZFP82 | ZFP82 zinc finger protein | NM_001321918.2 | 15.7% | 14.4% | (many diffs) |
| 5 | mouse | 330502 | Zfp82 | zinc finger protein 82 | NM_177889.5 | 82.6% | 85.1% | (many diffs) |
| 6 | mouse | 330502 | Zfp82 | zinc finger protein 82 | NM_001252519.1 | 77.7% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1662
- ORF length:
- 1596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccttcgatca gtgatgttca gtgatgtatc catagacttc tctccagaag 121 agtgggaata cctggacttg gaacaaaagg acttgtacag agatgtcatg ttggagaact 181 acagcaactt ggtctcactg ggatgcttca tttctaaacc agatgtgatt tcctcattgg 241 agcaaggaaa agagccttgg aaagttgtga ggaaaggaag aagacaatat ccagatttgg 301 agaccaagta tgagaccaag aagttatctt tagaaaatga catttatgaa ataaatttat 361 cccagtggaa gataatggaa agaattgaaa accatggcct taagggtctc attttaaaaa 421 atgattggga atccacagga aaaattgaag gacaggagag acctcaagaa ggatacttca 481 gtagtgtgaa aatgccatct gaaaaggtgt cctcttacca gaaacgcaca tctgttactc 541 cacatcagag acttcatttt gttgataaac cctatgaatg taaggaatgt gggaaggcgt 601 tcagagtgcg ccaacagctt acttttcatc acagaattca tactggtgaa aaaccgtatg 661 aatgtaagga atgtgggatg gccttcagac agactgcaca ccttactcga catcagagac 721 ttcattctgg tgaaaaactc tatgaatgta aggaatgtgg ggaagctttc atatgtggtg 781 cagatcttag agtacatcag aaaatgcata ttggtgagaa gccctatgaa tgtaaagaat 841 gtgggaaggc ttttagggta cgaggacaac ttactctgca tcagaggatt catactggtg 901 agaaacccta tgtgtgtaaa gagtgtggaa aagcctttag acagtacgca cacctgactc 961 ggcatcagaa gcttaatagt gctgacaggc tctatgaatg caaagaatgt gggaaggcct 1021 ttttgtgtgg ctctggtctt agagtacatc acaaacttca tactggtgag aaaccctatg 1081 aatgtaagga atgcgggaag gcctttagag tgcgacaaca actaacactc catcagagaa 1141 ttcatactgg tgagaaaccc tatgaatgta aggaatgtgg aaagaccttt agccgtggct 1201 atcatcttat tctccatcac agaattcata ctggtgaaaa accttacgaa tgtaaggaat 1261 gctggaaagc ctttagtcgc tactcacaac ttatttcaca tcagagtatt catattggtg 1321 ttaagcccta tgactgtaag gaatgcggga aggccttcag actactttca caactcacac 1381 agcatcagag tattcatatt ggtgagaaac cttataaatg taaggaatgt ggcaaggcct 1441 ttagattgcg ccaaaaactt actctacatc agagcattca tactggcgaa aaaccctttg 1501 agtgtaagga atgtaggaag gcctttagac ttaattcatc ccttattcaa catcTGAGAA 1561 TTCATTCTGG TGAGAAACCC TATGAATGTA AGGAATGTAA GAAGGCCTTT AGGCAACATT 1621 CACACCTTAC TCATCATCTG AAAATTCATA ATGTAAAAAT CTACCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGAGCT TTTTGACGAT CAATCTAGCA CACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t