Construct: ORF TRCN0000472963
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016966.1_s317c1
- Derived from:
- ccsbBroadEn_10959
- DNA Barcode:
- ACTTCTTGTGTCTCCCCCGAACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP6 (4135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472963
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4135 | MAP6 | microtubule associated prot... | XM_017017758.1 | 96.8% | 96.8% | 132_179del |
| 2 | human | 4135 | MAP6 | microtubule associated prot... | XM_017017757.1 | 94.5% | 94.5% | 1_84del |
| 3 | human | 4135 | MAP6 | microtubule associated prot... | XM_017017756.1 | 91.6% | 91.6% | 1_84del;216_263del |
| 4 | human | 4135 | MAP6 | microtubule associated prot... | NM_033063.1 | 59.5% | 59.5% | 1_987del |
| 5 | human | 4135 | MAP6 | microtubule associated prot... | XM_017017755.2 | 58.3% | 58.3% | 1_987del;1119_1166del |
| 6 | human | 4135 | MAP6 | microtubule associated prot... | NM_207577.1 | 13.5% | 13.5% | 1_987del;1317_1318ins1122 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1521
- ORF length:
- 1452
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttcatgag accagctaca gtgctcagtt caaaggagag gccagcaagc 121 caacaacagc tgacaataag gtcattgatc gcagaagaat acgcagcctc tacagcgaac 181 ccttcaagga acccccaaag gtggaaaaac ctagtgttca gagttccaaa ccaaaaaaga 241 cctcagcgag ccataagccc acgaggaagg ccaaagacaa gcaggcggtg tcaggccagg 301 ctgccaagaa aaagagcgcg gagggcccga gtaccaccaa gccagacgac aaggagcaaa 361 gcaaagagat gaacaataaa ctggctgagg cgaaagagag cctggctcaa cccgtcagtg 421 attcaagtaa gactcaaggt cctgtagcca cagagccaga caaggatcaa ggttctgtgg 481 tcccaggcct tctgaaaggt caaggtccta tggtgcaaga gcctctgaag aagcaaggtt 541 ctgtggtccc agggcctcca aaggatctag gtcccatgat cccattacca gtcaaggatc 601 aagatcacac ggtccctgag cctttaaaga atgaaagccc tgttatctca gcaccagtca 661 aggaccaagg tccctcggtc ccagttcctc caaagaatca aagtcctatg gttccagcaa 721 aagttaagga tcaaggctct gtggtaccag agtctctaaa ggatcaaggt cctaggattc 781 ctgagcctgt gaagaatcaa gctcctatgg tcccagcacc tgtcaaggat gaaggtccca 841 tggtctcagc atctgtcaag gatcaaggtc ccatggtctc agcacctgtc aaggatcaag 901 gtcccatagt cccagcacct gtcaagggtg aaggtcccat agtcccagca cctgtcaagg 961 atgaaggtcc catggtctca gcacctatca aggatcaaga tcccatggTC CCAGAGCATC 1021 CGAAGGATGA AAGTGCCATG GCCACAGCAC CCATAAAGAA TCAAGGTTCC ATGGTCTCTG 1081 AGCCTGTAAA GAATCAAGGT TTAGTGGTCT CAGGGCCAGT CAAGGATCAA GATGTTGTAG 1141 TCCCAGAGCA TGCAAAGGTT CACGATTCTG CAGTTGTGGC ACCTGTAAAG AATCAAGGTC 1201 CTGTGGTCCC CGAGTCCGTG AAGAATCAAG ACCCCATTCT CCCAGTACTA GTTAAGGATC 1261 AAGGCCCCAC AGTCCTACAG CCTCCAAAGA ATCAAGGTCG TATAGTCCCT GAACCTCTGA 1321 AGAATCAAGT TCCTATAGTC CCAGTGCCTC TGAAGGATCA AGATCCTCTG GTGCCAGTAC 1381 CAGCAAAGGA CCAAGGTCCT GCAGTCCCTG AACCTCTGAA GACTCAAGGT CCCAGGGACC 1441 CTCAGCTACC TACTGTCTCA CCTCTACCCC GAGTCATGAT CCCAACTGCC CCCCATACGG 1501 AATACATTGA GAGCTCCCCT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTT CTTGTGTCTC 1681 CCCCGAACAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt