Construct: ORF TRCN0000472965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014180.1_s317c1
- Derived from:
- ccsbBroadEn_00677
- DNA Barcode:
- CGGACGCTATGATTTTTACCGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR34 (2857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2857 | GPR34 | G protein-coupled receptor 34 | NM_001097579.2 | 100% | 100% | |
| 2 | human | 2857 | GPR34 | G protein-coupled receptor 34 | NM_005300.4 | 100% | 100% | |
| 3 | human | 2857 | GPR34 | G protein-coupled receptor 34 | XM_005272597.4 | 100% | 100% | |
| 4 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | NM_011823.4 | 85.6% | 89.5% | (many diffs) |
| 5 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | XM_006527624.3 | 85.6% | 89.5% | (many diffs) |
| 6 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | XM_006527625.3 | 85.6% | 89.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aagtcatacc ataacaatga cgacaacttc agtcagcagc tggccttact 121 cctcccacag aatgcgcttt ataaccaatc atagcgacca accgccacaa aacttctcag 181 caacaccaaa tgttactacc tgtcccatgg atgaaaaatt gctatctact gtgttaacca 241 catcctactc tgttattttc atcgtgggac tggttgggaa cataatcgcc ctctatgtat 301 ttctgggtat tcaccgtaaa agaaattcca ttcaaattta tctacttaac gtagccattg 361 cagacctcct actcatcttc tgcctccctt tccgaataat gtatcatatt aaccaaaaca 421 agtggacact aggtgtgatt ctgtgcaagg ttgtgggaac actgttttat atgaacatgt 481 acattagcat tattttgctt ggattcatca gtttggatcg ctatataaaa attaatcggt 541 ctatacagca acggaaggca ataacaacca aacaaagtat ttatgtctgt tgtatagtat 601 ggatgcttgc tcttggtgga ttcctaacta tgattatttt aacacttaag aaaggagggc 661 ataattccac aatgtgtttc caTTACAGAG ATAAGCATAA CGCAAAAGGA GAAGCCATTT 721 TTAACTTCAT TCTTGTGGTA ATGTTCTGGC TAATTTTCTT ACTAATAATC CTTTCATATA 781 TTAAGATTGG GAAGAATCTA TTGAGGATTT CTAAAAGGAG GTCAAAATTT CCTAATTCTG 841 GTAAATATGC CACTACAGCT CGTAACTCCT TTATTGTACT TATCATTTTT ACTATATGTT 901 TTGTTCCCTA TCATGCCTTT CGATTCATCT ACATTTCTTC ACAGCTAAAT GTATCATCTT 961 GCTACTGGAA AGAAATTGTT CACAAAACCA ATGAGATCAT GCTGGTTCTC TCATCTTTCA 1021 ATAGTTGCTT AGATCCAGTC ATGTATTTCC TGATGTCCAG TAACATTCGC AAAATAATGT 1081 GCCAACTTCT TTTTAGACGA TTTCAAGGTG AACCAAGTAG GAGTGAAAGC ACTTCAGAAT 1141 TTAAACCAGG ATACTCCCTG CATGATACAT CTGTGGCAGT GAAAATACAG TCTAGTTCTA 1201 AAAGTACTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGGACG CTATGATTTT TACCGCAAAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt